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銅置換ラットジペプチジルペプチダーゼIIIにおける銅イオンの配位構造の変化が酵素の活性発現に重要である。(発表論文抄録(2012))
https://fukuyama-u.repo.nii.ac.jp/records/8773
https://fukuyama-u.repo.nii.ac.jp/records/877335dc394c-cb7c-485f-81e9-1644d45178eb
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
廣瀬順造(発表論文抄録(2012)) (180.0 kB)
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Copyright (c) 2013 by Fukuyama University
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2017-12-20 | |||||
| タイトル | ||||||
| タイトル | 銅置換ラットジペプチジルペプチダーゼIIIにおける銅イオンの配位構造の変化が酵素の活性発現に重要である。(発表論文抄録(2012)) | |||||
| タイトル | ||||||
| タイトル | Flexibility of the coordination geometry around the cupric ions in Cu(II)-rat dipeptidyl peptidase III is important for the expression of enzyme activity. | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | 2-Naphthylamine | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Animals | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Copper | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Dipeptidyl-Peptidases and Tripeptidyl-Peptidases | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Models, Molecular | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Protein Conformation | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Rats | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Sequence Deletion | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
Hirose, Junzo
× Hirose, Junzo× Hata, Toshiyuki× Kawaoka, Chie× Ikeura, Tomohiro× Kitahara, Suguru× Tomida, Hisao× Iwamoto, Hiroyuki× Ono, Yukio× Fukasawa, Kayoko M |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 45879 | |||||
| 識別子Scheme | CiNii ID | |||||
| 識別子URI | http://ci.nii.ac.jp/nrid/1000070080215 | |||||
| 識別子 | 1000070080215 | |||||
| 姓名 | 廣瀬, 順造 | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 45236 | |||||
| 識別子Scheme | CiNii ID | |||||
| 識別子URI | http://ci.nii.ac.jp/nrid/9000014625803 | |||||
| 識別子 | 9000014625803 | |||||
| 姓名 | 秦, 季之 | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 45880 | |||||
| 姓名 | 川岡, 知恵 | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 45881 | |||||
| 姓名 | 池浦, 知宏 | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 45882 | |||||
| 姓名 | 北原, 卓 | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 45166 | |||||
| 識別子Scheme | CiNii ID | |||||
| 識別子URI | http://ci.nii.ac.jp/nrid/9000005174459 | |||||
| 識別子 | 9000005174459 | |||||
| 姓名 | 冨田, 久夫 | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 45883 | |||||
| 識別子Scheme | CiNii ID | |||||
| 識別子URI | http://ci.nii.ac.jp/nrid/9000004407540 | |||||
| 識別子 | 9000004407540 | |||||
| 姓名 | 岩本, 博行 | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 45238 | |||||
| 識別子Scheme | CiNii ID | |||||
| 識別子URI | http://ci.nii.ac.jp/nrid/9000002578762 | |||||
| 識別子 | 9000002578762 | |||||
| 姓名 | 小野, 行雄 | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 45884 | |||||
| 識別子Scheme | CiNii ID | |||||
| 識別子URI | http://ci.nii.ac.jp/nrid/9000000181432 | |||||
| 識別子 | 9000000181432 | |||||
| 姓名 | 深沢, 加与子 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Dipeptidyl peptidase III (DPP III), the zinc peptidase, has a unique helix portion in the metal-binding motif (HELLGH). The enzyme activity of the cupric derivative of rat DPP III (Cu(II)-rat DPP III) for Lys-Ala-β-NA is about 30% of that of the wild-type enzyme. On the other hand, the enzyme activity of Cu(II)-rat del-DPP III, in which Leu453 is deleted from the metal-binding motif, possesses only 1-2% of the enzyme activity of rat del-DPP III. The EPR spectra of Cu(II)-rat DPP III in the presence of various concentrations of the substrate, Lys-Ala-β-NA, changed dramatically, showing formation of the enzyme-metal-substrate complex. The EPR spectra of Cu(II)-rat del-DPP III did not change in the presence of excess Lys-Ala-β-NA. The deletion of Leu453 from the HELLGH motif of rat DPP III leads to a complete loss of flexibility in the ligand geometry around the cupric ions. Under the formation of the enzyme-metal-substrate complex, Glu451 of Cu(II)-rat DPP III is sufficiently able to approach the water molecule via a very different orientation from that of the resting state; however, Glu451 of Cu(II)-rat del-DPP III is not able to access the water molecule. | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Dipeptidyl peptidase III (DPP III), the zinc peptidase, has a unique helix portion in the metal-binding motif (HELLGH). The enzyme activity of the cupric derivative of rat DPP III (Cu(II)-rat DPP III) for Lys-Ala-β-NA is about 30% of that of the wild-type enzyme. On the other hand, the enzyme activity of Cu(II)-rat del-DPP III, in which Leu453 is deleted from the metal-binding motif, possesses only 1-2% of the enzyme activity of rat del-DPP III. The EPR spectra of Cu(II)-rat DPP III in the presence of various concentrations of the substrate, Lys-Ala-β-NA, changed dramatically, showing formation of the enzyme-metal-substrate complex. The EPR spectra of Cu(II)-rat del-DPP III did not change in the presence of excess Lys-Ala-β-NA. The deletion of Leu453 from the HELLGH motif of rat DPP III leads to a complete loss of flexibility in the ligand geometry around the cupric ions. Under the formation of the enzyme-metal-substrate complex, Glu451 of Cu(II)-rat DPP III is sufficiently able to approach the water molecule via a very different orientation from that of the resting state; however, Glu451 of Cu(II)-rat del-DPP III is not able to access the water molecule. | |||||
| 書誌情報 |
福山大学薬学部研究年報 en : Annual report of the Faculty of Pharmacy & Pharmaceutical Sciences, Fukuyama University 号 31, p. 37-38, 発行日 2013-12-25 |
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| 出版者 | ||||||
| 出版者 | 福山大学薬学部 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0288-724X | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN10064550 | |||||
