{"created":"2023-06-19T09:51:34.310033+00:00","id":8773,"links":{},"metadata":{"_buckets":{"deposit":"e50786b6-4564-4c7b-92fc-13a7dbe7baaa"},"_deposit":{"created_by":18,"id":"8773","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"8773"},"status":"published"},"_oai":{"id":"oai:fukuyama-u.repo.nii.ac.jp:00008773","sets":["502:503:627:783"]},"author_link":["45238","45877","45166","45872","45871","45876","45874","45873","45880","45882","45884","45883","45878","45879","45875","45236","45870","45881"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-12-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"31","bibliographicPageEnd":"38","bibliographicPageStart":"37","bibliographic_titles":[{"bibliographic_title":"福山大学薬学部研究年報"},{"bibliographic_title":"Annual report of the Faculty of Pharmacy & Pharmaceutical Sciences, Fukuyama University","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Dipeptidyl peptidase III (DPP III), the zinc peptidase, has a unique helix portion in the metal-binding motif (HELLGH). The enzyme activity of the cupric derivative of rat DPP III (Cu(II)-rat DPP III) for Lys-Ala-β-NA is about 30% of that of the wild-type enzyme. On the other hand, the enzyme activity of Cu(II)-rat del-DPP III, in which Leu453 is deleted from the metal-binding motif, possesses only 1-2% of the enzyme activity of rat del-DPP III. The EPR spectra of Cu(II)-rat DPP III in the presence of various concentrations of the substrate, Lys-Ala-β-NA, changed dramatically, showing formation of the enzyme-metal-substrate complex. The EPR spectra of Cu(II)-rat del-DPP III did not change in the presence of excess Lys-Ala-β-NA. The deletion of Leu453 from the HELLGH motif of rat DPP III leads to a complete loss of flexibility in the ligand geometry around the cupric ions. Under the formation of the enzyme-metal-substrate complex, Glu451 of Cu(II)-rat DPP III is sufficiently able to approach the water molecule via a very different orientation from that of the resting state; however, Glu451 of Cu(II)-rat del-DPP III is not able to access the water molecule.","subitem_description_type":"Abstract"}]},"item_10002_description_6":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Dipeptidyl peptidase III (DPP III), the zinc peptidase, has a unique helix portion in the metal-binding motif (HELLGH). The enzyme activity of the cupric derivative of rat DPP III (Cu(II)-rat DPP III) for Lys-Ala-β-NA is about 30% of that of the wild-type enzyme. On the other hand, the enzyme activity of Cu(II)-rat del-DPP III, in which Leu453 is deleted from the metal-binding motif, possesses only 1-2% of the enzyme activity of rat del-DPP III. The EPR spectra of Cu(II)-rat DPP III in the presence of various concentrations of the substrate, Lys-Ala-β-NA, changed dramatically, showing formation of the enzyme-metal-substrate complex. The EPR spectra of Cu(II)-rat del-DPP III did not change in the presence of excess Lys-Ala-β-NA. The deletion of Leu453 from the HELLGH motif of rat DPP III leads to a complete loss of flexibility in the ligand geometry around the cupric ions. Under the formation of the enzyme-metal-substrate complex, Glu451 of Cu(II)-rat DPP III is sufficiently able to approach the water molecule via a very different orientation from that of the resting state; however, Glu451 of Cu(II)-rat del-DPP III is not able to access the water molecule.","subitem_description_type":"Other"}]},"item_10002_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"45879","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000070080215","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000070080215"}],"names":[{"name":"廣瀬, 順造"}]},{"nameIdentifiers":[{"nameIdentifier":"45236","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000014625803","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000014625803"}],"names":[{"name":"秦, 季之"}]},{"nameIdentifiers":[{"nameIdentifier":"45880","nameIdentifierScheme":"WEKO"}],"names":[{"name":"川岡, 知恵"}]},{"nameIdentifiers":[{"nameIdentifier":"45881","nameIdentifierScheme":"WEKO"}],"names":[{"name":"池浦, 知宏"}]},{"nameIdentifiers":[{"nameIdentifier":"45882","nameIdentifierScheme":"WEKO"}],"names":[{"name":"北原, 卓"}]},{"nameIdentifiers":[{"nameIdentifier":"45166","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000005174459","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000005174459"}],"names":[{"name":"冨田, 久夫"}]},{"nameIdentifiers":[{"nameIdentifier":"45883","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000004407540","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000004407540"}],"names":[{"name":"岩本, 博行"}]},{"nameIdentifiers":[{"nameIdentifier":"45238","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000002578762","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000002578762"}],"names":[{"name":"小野, 行雄"}]},{"nameIdentifiers":[{"nameIdentifier":"45884","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000000181432","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000000181432"}],"names":[{"name":"深沢, 加与子"}]}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"福山大学薬学部"}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN10064550","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0288-724X","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hirose, Junzo"}],"nameIdentifiers":[{"nameIdentifier":"45870","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hata, Toshiyuki"}],"nameIdentifiers":[{"nameIdentifier":"45871","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kawaoka, Chie"}],"nameIdentifiers":[{"nameIdentifier":"45872","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ikeura, Tomohiro"}],"nameIdentifiers":[{"nameIdentifier":"45873","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kitahara, Suguru"}],"nameIdentifiers":[{"nameIdentifier":"45874","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tomida, Hisao"}],"nameIdentifiers":[{"nameIdentifier":"45875","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Iwamoto, Hiroyuki"}],"nameIdentifiers":[{"nameIdentifier":"45876","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ono, Yukio"}],"nameIdentifiers":[{"nameIdentifier":"45877","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fukasawa, Kayoko M"}],"nameIdentifiers":[{"nameIdentifier":"45878","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-12-20"}],"displaytype":"detail","filename":"31-37.pdf","filesize":[{"value":"180.0 kB"}],"format":"application/pdf","license_note":"Copyright (c) 2013 by Fukuyama University","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"廣瀬順造(発表論文抄録(2012))","url":"https://fukuyama-u.repo.nii.ac.jp/record/8773/files/31-37.pdf"},"version_id":"2e4df173-009f-48ee-83e3-0b240194a0d3"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"2-Naphthylamine","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Animals","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Copper","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Dipeptidyl-Peptidases and Tripeptidyl-Peptidases","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Models, Molecular","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Protein Conformation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Rats","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Sequence Deletion","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"銅置換ラットジペプチジルペプチダーゼIIIにおける銅イオンの配位構造の変化が酵素の活性発現に重要である。(発表論文抄録(2012))","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"銅置換ラットジペプチジルペプチダーゼIIIにおける銅イオンの配位構造の変化が酵素の活性発現に重要である。(発表論文抄録(2012))"},{"subitem_title":"Flexibility of the coordination geometry around the cupric ions in Cu(II)-rat dipeptidyl peptidase III is important for the expression of enzyme activity.","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"18","path":["783"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-12-20"},"publish_date":"2017-12-20","publish_status":"0","recid":"8773","relation_version_is_last":true,"title":["銅置換ラットジペプチジルペプチダーゼIIIにおける銅イオンの配位構造の変化が酵素の活性発現に重要である。(発表論文抄録(2012))"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-06-19T10:17:16.403548+00:00"}