WEKO3
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ロイコトリエンB_4ω水酸化酵素の研究
https://fukuyama-u.repo.nii.ac.jp/records/7970
https://fukuyama-u.repo.nii.ac.jp/records/79705daaf5d4-c22b-441a-b507-b179bfa61fe8
名前 / ファイル | ライセンス | アクション |
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KJ00005781369.pdf (793.1 kB)
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Item type | 紀要論文(ELS) / Departmental Bulletin Paper(1) | |||||
---|---|---|---|---|---|---|
公開日 | 1996-03-01 | |||||
タイトル | ||||||
タイトル | ロイコトリエンB_4ω水酸化酵素の研究 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Studies on Leukotriene B_4 ω-Hydroxylase | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 脂肪酸ω水酸化酵素 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | チトクロームP450 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ロイコトリエンB_4ω水酸化酵素 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | CYP4F | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cDNA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | クローニング | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 遺伝子 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 染色体 | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ページ属性 | ||||||
内容記述タイプ | Other | |||||
内容記述 | P(論文) | |||||
著者名(日) |
楠瀬, 正道
× 楠瀬, 正道× 菊田, 安至 |
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著者名よみ | ||||||
識別子Scheme | WEKO | |||||
識別子 | 42828 | |||||
姓名 | クスノセ, マサミチ | |||||
著者名よみ | ||||||
識別子Scheme | WEKO | |||||
識別子 | 42829 | |||||
姓名 | キクタ, ヤスシ | |||||
著者名(英) | ||||||
識別子Scheme | WEKO | |||||
識別子 | 42830 | |||||
姓名 | KUSUNOSE, Masamichi | |||||
言語 | en | |||||
著者名(英) | ||||||
識別子Scheme | WEKO | |||||
識別子 | 42831 | |||||
姓名 | KIKUTA, Yasushi | |||||
言語 | en | |||||
著者所属(日) | ||||||
値 | 福山大学工学部食品工学科 | |||||
著者所属(日) | ||||||
値 | 福山大学工学部食品工学科 | |||||
抄録(英) | ||||||
内容記述タイプ | Other | |||||
内容記述 | We have isolated and sequenced cDNAs encoding two human leukotriene B_4 (LTB_4) ω-hydroxylases. Human neutrophil enzyme (CYP4F3) contained 520 amino acids with a molecular weight of 59,805 Da, while human liver enzyme (CYP4F2) contained 520 amino acids with a molecular weight of 59,853 Da. The two enzymes showed 87.3% sequence homology with each other. The microsomes from yeast cells transfected with the cDNAs for CYP4F3 and CYP4F2 catalyzed the ω-hydroxylation of LTB_4 with Km values of 0.71μM and 46μM, respectively. Highly purified enzyme of CYP4F3 also exhibited ω-hydroxylase activities toward LTB_4, 6-trans-LTB_4, 20-hydroxy LTB_4, lipoxin A_4, lipoxin B_4, and 12-HETE. Sourthern blot of human genomic DNA probed with the CYP4F3 cDNA indicated the existence of at least 5 related genes of CYP4F. The CYP4F3 gene, which contained 12 exons, was mapped to chromosome 19p13.2. | |||||
雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00217655 | |||||
書誌情報 |
福山大学工学部紀要 巻 19, 号 2, p. 61-69, 発行日 1996-03 |