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藍藻Synechocystis sp. SY-4の凍結保存法の検討
https://fukuyama-u.repo.nii.ac.jp/records/5317
https://fukuyama-u.repo.nii.ac.jp/records/53177c57fa50-83cb-4d71-b16f-bfdb6d702077
名前 / ファイル | ライセンス | アクション |
---|---|---|
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Item type | 紀要論文(ELS) / Departmental Bulletin Paper(1) | |||||
---|---|---|---|---|---|---|
公開日 | 1998-12-01 | |||||
タイトル | ||||||
タイトル | 藍藻Synechocystis sp. SY-4の凍結保存法の検討 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Cryopreservation of Blue-green Alga Synechocystis sp. SY-4 | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Synechocystis sp. | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | cryopreservation | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | trehalose | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | DMSO | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ページ属性 | ||||||
内容記述タイプ | Other | |||||
内容記述 | P(論文) | |||||
記事種別(日) | ||||||
値 | 原著論文 | |||||
記事種別(英) | ||||||
言語 | en | |||||
値 | Original | |||||
著者名(日) |
阪本, 憲司
× 阪本, 憲司× 北嶋, 克章× 雨村, 明倫 |
|||||
著者名よみ | ||||||
識別子Scheme | WEKO | |||||
識別子 | 26243 | |||||
姓名 | サカモト, ケンジ | |||||
著者名よみ | ||||||
識別子Scheme | WEKO | |||||
識別子 | 26244 | |||||
姓名 | キタジマ, カツアキ | |||||
著者名よみ | ||||||
識別子Scheme | WEKO | |||||
識別子 | 26245 | |||||
姓名 | アメムラ, アキノリ | |||||
著者名(英) | ||||||
識別子Scheme | WEKO | |||||
識別子 | 26246 | |||||
姓名 | Sakamoto, Kenji | |||||
言語 | en | |||||
著者名(英) | ||||||
識別子Scheme | WEKO | |||||
識別子 | 26247 | |||||
姓名 | Kitajima, Katsuaki | |||||
言語 | en | |||||
著者名(英) | ||||||
識別子Scheme | WEKO | |||||
識別子 | 26248 | |||||
姓名 | Amemura, Akinori | |||||
言語 | en | |||||
著者所属(日) | ||||||
値 | 海洋生物工学科:福山大学内海生物資源研究所 | |||||
著者所属(日) | ||||||
値 | 福山大学内海生物資源研究所 | |||||
著者所属(日) | ||||||
値 | 海洋生物工学科:福山大学内海生物資源研究所 | |||||
著者所属(英) | ||||||
言語 | en | |||||
値 | Department of Marine Biotechnology, Fukuyama University:Research Institute of Marine Bioresouces, Fukuyama Universitry | |||||
著者所属(英) | ||||||
言語 | en | |||||
値 | Research Institute of Marine Bioresouces, Fukuyama Universitry | |||||
著者所属(英) | ||||||
言語 | en | |||||
値 | Department of Marine Biotechnology, Fukuyama University:Research Institute of Marine Bioresouces, Fukuyama Universitry | |||||
抄録(日) | ||||||
内容記述タイプ | Other | |||||
内容記述 | 藍藻Synechocystis sp. SY-4(以下,SY-4と略す)は,本研究所において単離した微細藻である。SY-4は海産魚の種苗生産において初期餌料として用いられているシオミズツポワムシ(以下,ワムシと略す)の培養用餌料に好適であり,水温35℃でも増殖可能な耐高温性を有するため,夏期における餌料不足を補うことができると期待される。しかし,水温20℃以下の培養が不調であり冬期の屋外培養が困難となるため,この期間の保存が必要となる。そこで本研究では,元種保存を目的としたSY-4の凍結保存について検討した。 | |||||
抄録(英) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Cryopreservation of blue-green alga Synechocystis sp. SY-4 was examined. Synechocystis sp. SY-4 was frozen with high levels of post-thaw relative growth activity (more than 100%), which were achieved by employing a simple protocol using trehalose or DMSO as a cryoprotectant; samples were placed in a cooling-bath preset at -40℃ for 30 min, followed by liquid nitrogen freezing. The highest level of relative growth activity (141% or 124%) after freezing and thawing was obtained using cultures at early-stationary phase grown at 25℃, and cryoprotectant of 5% (w/v) trehalose or 7.5% (v/v) DMSO, respectively. Furthermore, the levels for cells cryopreserved in liquid nitrogen for 2 months were 70% and 120% when 5% trehalose and 7.5% DMSO were used, respectively. | |||||
雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA1157543X | |||||
書誌情報 |
福山大学内海生物資源研究所報告 巻 9, p. 25-32, 発行日 1998-12 |