@article{oai:fukuyama-u.repo.nii.ac.jp:00005317,
 author = {阪本, 憲司 and 北嶋, 克章 and 雨村, 明倫},
 journal = {福山大学内海生物資源研究所報告},
 month = {Dec},
 note = {P(論文), 藍藻Synechocystis sp. SY-4(以下,SY-4と略す)は,本研究所において単離した微細藻である。SY-4は海産魚の種苗生産において初期餌料として用いられているシオミズツポワムシ(以下,ワムシと略す)の培養用餌料に好適であり,水温35℃でも増殖可能な耐高温性を有するため,夏期における餌料不足を補うことができると期待される。しかし,水温20℃以下の培養が不調であり冬期の屋外培養が困難となるため,この期間の保存が必要となる。そこで本研究では,元種保存を目的としたSY-4の凍結保存について検討した。, Cryopreservation of blue-green alga Synechocystis sp. SY-4 was examined. Synechocystis sp. SY-4 was frozen with high levels of post-thaw relative growth activity (more than 100%), which were achieved by employing a simple protocol using trehalose or DMSO as a cryoprotectant; samples were placed in a cooling-bath preset at -40℃ for 30 min, followed by liquid nitrogen freezing. The highest level of relative growth activity (141% or 124%) after freezing and thawing was obtained using cultures at early-stationary phase grown at 25℃, and cryoprotectant of 5% (w/v) trehalose or 7.5% (v/v) DMSO, respectively. Furthermore, the levels for cells cryopreserved in liquid nitrogen for 2 months were 70% and 120% when 5% trehalose and 7.5% DMSO were used, respectively.},
 pages = {25--32},
 title = {藍藻Synechocystis sp. SY-4の凍結保存法の検討},
 volume = {9},
 year = {1998}
}