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蛍光誘導体化試薬4-(5,6-ジメトキシ-2-フタルイミジニル)-2-メトキシフェニルスルホニルクロリドを用いる尿中カルニシンの高速液体クロマトグラフ法による高感度定量(発表論文抄録(2009))
https://fukuyama-u.repo.nii.ac.jp/records/8600
https://fukuyama-u.repo.nii.ac.jp/records/8600ea437409-de9a-4ae0-9386-e9744cfbe09e
名前 / ファイル | ライセンス | アクション |
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Copyright (c) 2010 by Fukuyama
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2017-01-28 | |||||
タイトル | ||||||
タイトル | 蛍光誘導体化試薬4-(5,6-ジメトキシ-2-フタルイミジニル)-2-メトキシフェニルスルホニルクロリドを用いる尿中カルニシンの高速液体クロマトグラフ法による高感度定量(発表論文抄録(2009)) | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Sensitive determination of carnosine in urine by high-performance liquid chromatography using 4-(5,6-dimethoxy-2-phthalimidinyl)-2-methoxyphenylsulfonyl chloride as a fluorescent labeling reagent. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Adult | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Calibration | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Carnosine | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Chromatography, High Pressure Liquid | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Female | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Fluorescent Dyes | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Humans | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Hydrolysis | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Indicators and Reagents | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Limit of Detection | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Male | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Phthalimides | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Reference Standards | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Temperature | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Time Factors | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Young Adult | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
鶴田, 泰人
× 鶴田, 泰人× 丸山, 潔× 井上, 裕文× 古謝, 景子× 伊達, 有子× 岡村, 信幸× 江藤, 精二× 小嶋, 英二朗 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A simple and highly sensitive high-performance liquid chromatography procedure was developed for the determination of carnosine in urine. Carnosine was derivatized with 4-(5,6-dimethoxy-2-phthalimidinyl)-2-methoxyphenylsulfonyl chloride at 70 degrees C for 15 min in borate buffer (20 mmol l(-1), pH 9.0) to produce fluorescent sulfonamides. After hydrolysis of the reaction mixture with formic acid at 100 degrees C for 15 min, the fluorescent derivative of carnosine was separated on a reversed-phase column with a linear gradient elution using solvents of (A) acetate buffer (0.1 mmol l(-1), pH 7.0) and (B) acetonitrile at a flow-rate of 1.0 ml/min and was detected at excitation and emission wavelengths of 318 and 400 nm, respectively. The detection limit of carnosine was 4 fmol at a signal-to-noise ratio of 3. The within-day and day-to-day relative standard deviations were 2.7-4.6% and 0.4-5.2%, respectively. The concentration of carnosine in normal human urine was found to be 4.6-125 nmol (mg creatinine)(-1) (mean+/-SD: 21.6+/-26.6 nmol (mg creatinine)(-1), n=20). | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | A simple and highly sensitive high-performance liquid chromatography procedure was developed for the determination of carnosine in urine. Carnosine was derivatized with 4-(5,6-dimethoxy-2-phthalimidinyl)-2-methoxyphenylsulfonyl chloride at 70 degrees C for 15 min in borate buffer (20 mmol l(-1), pH 9.0) to produce fluorescent sulfonamides. After hydrolysis of the reaction mixture with formic acid at 100 degrees C for 15 min, the fluorescent derivative of carnosine was separated on a reversed-phase column with a linear gradient elution using solvents of (A) acetate buffer (0.1 mmol l(-1), pH 7.0) and (B) acetonitrile at a flow-rate of 1.0 ml/min and was detected at excitation and emission wavelengths of 318 and 400 nm, respectively. The detection limit of carnosine was 4 fmol at a signal-to-noise ratio of 3. The within-day and day-to-day relative standard deviations were 2.7-4.6% and 0.4-5.2%, respectively. The concentration of carnosine in normal human urine was found to be 4.6-125 nmol (mg creatinine)(-1) (mean+/-SD: 21.6+/-26.6 nmol (mg creatinine)(-1), n=20). | |||||
書誌情報 |
福山大学薬学部研究年報 en : Annual report of the Faculty of Pharmacy & Pharmaceutical Sciences, Fukuyama University 号 28, p. 51-51, 発行日 2010-12-25 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0288-724X | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN10064550 |