{"_buckets": {"deposit": "dd862827-9399-42ba-bca4-ee635c4526f3"}, "_deposit": {"created_by": 18, "id": "8600", "owners": [18], "pid": {"revision_id": 0, "type": "depid", "value": "8600"}, "status": "published"}, "_oai": {"id": "oai:fukuyama-u.repo.nii.ac.jp:00008600", "sets": ["763"]}, "author_link": ["45338", "45101", "45337", "45108", "45336", "45339", "45106", "45335"], "item_10002_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2010-12-25", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "28", "bibliographicPageEnd": "51", "bibliographicPageStart": "51", "bibliographic_titles": [{"bibliographic_title": "福山大学薬学部研究年報"}, {"bibliographic_title": "Annual report of the Faculty of Pharmacy \u0026 Pharmaceutical Sciences, Fukuyama University", "bibliographic_titleLang": "en"}]}]}, "item_10002_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "A simple and highly sensitive high-performance liquid chromatography procedure was developed for the determination of carnosine in urine. Carnosine was derivatized with 4-(5,6-dimethoxy-2-phthalimidinyl)-2-methoxyphenylsulfonyl chloride at 70 degrees C for 15 min in borate buffer (20 mmol l(-1), pH 9.0) to produce fluorescent sulfonamides. After hydrolysis of the reaction mixture with formic acid at 100 degrees C for 15 min, the fluorescent derivative of carnosine was separated on a reversed-phase column with a linear gradient elution using solvents of (A) acetate buffer (0.1 mmol l(-1), pH 7.0) and (B) acetonitrile at a flow-rate of 1.0 ml/min and was detected at excitation and emission wavelengths of 318 and 400 nm, respectively. The detection limit of carnosine was 4 fmol at a signal-to-noise ratio of 3. The within-day and day-to-day relative standard deviations were 2.7-4.6% and 0.4-5.2%, respectively. The concentration of carnosine in normal human urine was found to be 4.6-125 nmol (mg creatinine)(-1) (mean+/-SD: 21.6+/-26.6 nmol (mg creatinine)(-1), n=20).", "subitem_description_type": "Abstract"}]}, "item_10002_description_6": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "A simple and highly sensitive high-performance liquid chromatography procedure was developed for the determination of carnosine in urine. Carnosine was derivatized with 4-(5,6-dimethoxy-2-phthalimidinyl)-2-methoxyphenylsulfonyl chloride at 70 degrees C for 15 min in borate buffer (20 mmol l(-1), pH 9.0) to produce fluorescent sulfonamides. After hydrolysis of the reaction mixture with formic acid at 100 degrees C for 15 min, the fluorescent derivative of carnosine was separated on a reversed-phase column with a linear gradient elution using solvents of (A) acetate buffer (0.1 mmol l(-1), pH 7.0) and (B) acetonitrile at a flow-rate of 1.0 ml/min and was detected at excitation and emission wavelengths of 318 and 400 nm, respectively. The detection limit of carnosine was 4 fmol at a signal-to-noise ratio of 3. The within-day and day-to-day relative standard deviations were 2.7-4.6% and 0.4-5.2%, respectively. The concentration of carnosine in normal human urine was found to be 4.6-125 nmol (mg creatinine)(-1) (mean+/-SD: 21.6+/-26.6 nmol (mg creatinine)(-1), n=20).", "subitem_description_type": "Other"}]}, "item_10002_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AN10064550", "subitem_source_identifier_type": "NCID"}]}, "item_10002_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0288-724X", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "鶴田, 泰人"}], "nameIdentifiers": [{"nameIdentifier": "45106", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9000005174471", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/9000005174471"}]}, {"creatorNames": [{"creatorName": "丸山, 潔"}], "nameIdentifiers": [{"nameIdentifier": "45335", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "井上, 裕文"}], "nameIdentifiers": [{"nameIdentifier": "45108", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9000006296797", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/9000006296797"}]}, {"creatorNames": [{"creatorName": "古謝, 景子"}], "nameIdentifiers": [{"nameIdentifier": "45336", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "伊達, 有子"}], "nameIdentifiers": [{"nameIdentifier": "45337", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9000005189599", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/9000005189599"}]}, {"creatorNames": [{"creatorName": "岡村, 信幸"}], "nameIdentifiers": [{"nameIdentifier": "45101", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "1000060169141", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/1000060169141"}]}, {"creatorNames": [{"creatorName": "江藤, 精二"}], "nameIdentifiers": [{"nameIdentifier": "45338", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9000002858230", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/9000002858230"}]}, {"creatorNames": [{"creatorName": "小嶋, 英二朗"}], "nameIdentifiers": [{"nameIdentifier": "45339", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9000321416466", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/9000321416466"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-01-28"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "蛍光誘導体化試薬4-(5,6-ジメトキシ-2-フタルイミジニル)-2-メトキシフェニルスルホニルクロリドを用いる尿中カルニシンの高速液体クロマトグラフ法による高感度定量.pdf", "filesize": [{"value": "496.0 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "Copyright (c) 2010 by Fukuyama ", "licensetype": "license_free", "mimetype": "application/pdf", "size": 496000.0, "url": {"label": "蛍光誘導体化試薬4-(5,6-ジメトキシ-2-フタルイミジニル)-2-メトキシフェニルスルホニルクロリドを用いる尿中カルニシンの高速液体クロマトグラフ法による高感度定量", "url": "https://fukuyama-u.repo.nii.ac.jp/record/8600/files/蛍光誘導体化試薬4-(5,6-ジメトキシ-2-フタルイミジニル)-2-メトキシフェニルスルホニルクロリドを用いる尿中カルニシンの高速液体クロマトグラフ法による高感度定量.pdf"}, "version_id": "fb496aa2-1214-44c8-a480-3a5dfb5c6829"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Adult", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Calibration", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Carnosine", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Chromatography, High Pressure Liquid", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Female", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Fluorescent Dyes", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Humans", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Hydrolysis", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Indicators and Reagents", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Limit of Detection", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Male", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Phthalimides", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Reference Standards", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Temperature", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Time Factors", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Young Adult", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "蛍光誘導体化試薬4-(5,6-ジメトキシ-2-フタルイミジニル)-2-メトキシフェニルスルホニルクロリドを用いる尿中カルニシンの高速液体クロマトグラフ法による高感度定量(発表論文抄録(2009))", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "蛍光誘導体化試薬4-(5,6-ジメトキシ-2-フタルイミジニル)-2-メトキシフェニルスルホニルクロリドを用いる尿中カルニシンの高速液体クロマトグラフ法による高感度定量(発表論文抄録(2009))"}, {"subitem_title": "Sensitive determination of carnosine in urine by high-performance liquid chromatography using 4-(5,6-dimethoxy-2-phthalimidinyl)-2-methoxyphenylsulfonyl chloride as a fluorescent labeling reagent.", "subitem_title_language": "en"}]}, "item_type_id": "10002", "owner": "18", "path": ["763"], "permalink_uri": "https://fukuyama-u.repo.nii.ac.jp/records/8600", "pubdate": {"attribute_name": "公開日", "attribute_value": "2017-01-28"}, "publish_date": "2017-01-28", "publish_status": "0", "recid": "8600", "relation": {}, "relation_version_is_last": true, "title": ["蛍光誘導体化試薬4-(5,6-ジメトキシ-2-フタルイミジニル)-2-メトキシフェニルスルホニルクロリドを用いる尿中カルニシンの高速液体クロマトグラフ法による高感度定量(発表論文抄録(2009))"], "weko_shared_id": -1}