WEKO3
インデックスリンク
アイテム
澱粉枝切り酵素プルラナーゼとシクロデキストリンとの相互作用
https://fukuyama-u.repo.nii.ac.jp/records/7971
https://fukuyama-u.repo.nii.ac.jp/records/7971a7befed3-dca5-4951-90ef-57cf73eb0883
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00005781370.pdf (708.4 kB)
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| Item type | 紀要論文(ELS) / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 1996-03-01 | |||||
| タイトル | ||||||
| タイトル | 澱粉枝切り酵素プルラナーゼとシクロデキストリンとの相互作用 | |||||
| タイトル | ||||||
| タイトル | Interaction of Starch-debranching-enzyme Pullulanase with Cyclodextrins | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Starch-debranching-enzyme | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Pullulanase | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Cyclodextrin | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Enzyme Kinetics | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Fluorometric Analysis | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| ページ属性 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | P(論文) | |||||
| 著者名(日) |
岩本, 博行
× 岩本, 博行× 廣瀬, 順造× 廣海, 啓太郎 |
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| 著者名よみ | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 42835 | |||||
| 姓名 | イワモト, ヒロユキ | |||||
| 著者名よみ | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 42836 | |||||
| 姓名 | ヒロセ, ジュンゾウ | |||||
| 著者名よみ | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 42837 | |||||
| 姓名 | ヒロミ, ケイタロウ | |||||
| 著者名(英) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 42838 | |||||
| 姓名 | IWAMOTO, Hiroyuki | |||||
| 言語 | en | |||||
| 著者名(英) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 42839 | |||||
| 姓名 | HIROSE, Junzo | |||||
| 言語 | en | |||||
| 著者名(英) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 42840 | |||||
| 姓名 | HIROMI, Keitaro | |||||
| 言語 | en | |||||
| 著者所属(日) | ||||||
| 値 | 福山大学工学部食品工学科 | |||||
| 著者所属(日) | ||||||
| 値 | 福山大学工学部食品工学科 | |||||
| 著者所属(日) | ||||||
| 値 | 福山大学工学部食品工学科 | |||||
| 抄録(英) | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Starch-debranching-enzyme hydrolyzes the α-1,6-glucosidic linkages of starch and other α-glucans. Isoamyase and pullulanase belong to this enzyme group, and are key enzymes for the industrial production of glucose, maltose and other oligosaccharides from starch. In this paper, we examined the interaction between pullulanase from Klebsiella pneumomae and α-, β-, and γ-cyclodextrins by means of inhibition studies of the enzyme activity, UV and fluorescence difference spectroscopy, and flow calorimetry. All the above cyclodextrins were found to be competitive inhibitors, but the inhibition and dissociation constants of β-cyclodextrin were two orders of magnitude less than those of α- and γ-cyclodextrins. The UV and fluorescence spectral change (excitation at 295 nm) induced by the addition of β-cyclodextrin were significantly different from those of other cyclodextrins. In the calorimetric analysis, only β-cyclodextrin showed a positive entropy change upon binding with the enzyme. Moreover, preliminary kinetic studies were done on the binding of the enzyme with cyclodextrins by following the increase of the fluorescence intensity with a micro-stopped-flow apparatus. The apparent first-order rate constants, K_<app>, for α- and γ-cyclodextrins were independent of the concentration of cyclodextrins. In contast, β-cyclodextrin showed a hyperbolic cyclodextrin concentration dependence of K_<app>. judged from these results, the binding mode of β-cyclodextrin is subtly different from those of α- and γ-cyclodextrins. | |||||
| 雑誌書誌ID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00217655 | |||||
| 書誌情報 |
福山大学工学部紀要 巻 19, 号 2, p. 71-80, 発行日 1996-03 |
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