{"_buckets": {"deposit": "69dcc64c-ac9d-40e3-bafd-df1c3401d57c"}, "_deposit": {"created_by": 3, "id": "7431", "owners": [3], "pid": {"revision_id": 0, "type": "depid", "value": "7431"}, "status": "published"}, "_oai": {"id": "oai:fukuyama-u.repo.nii.ac.jp:00007431", "sets": ["657"]}, "author_link": ["40866", "40863", "40870", "40867", "40869", "40864", "40862", "40865", "40868"], "item_1_biblio_info_14": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1993-12", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "12", "bibliographicPageStart": "1", "bibliographicVolumeNumber": "4", "bibliographic_titles": [{"bibliographic_title": "福山大学附属内海生物資源研究所報告"}]}]}, "item_1_creator_6": {"attribute_name": "著者名(日)", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "吉田, 美夫"}], "nameIdentifiers": [{"nameIdentifier": "40862", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "沖増, 英治"}], "nameIdentifiers": [{"nameIdentifier": "40863", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "雨村, 明倫"}], "nameIdentifiers": [{"nameIdentifier": "40864", "nameIdentifierScheme": "WEKO"}]}]}, "item_1_description_1": {"attribute_name": "ページ属性", "attribute_value_mlt": [{"subitem_description": "P(論文)", "subitem_description_type": "Other"}]}, "item_1_description_11": {"attribute_name": "抄録(日)", "attribute_value_mlt": [{"subitem_description": "ニジマス白血球の15-LO酵素の分子量は約70,000であり,哺乳類の15-LO酵素の分子量とも類似していた。至適反応pHは7.5であり,これも至適反応pH7.0をもつ哺乳類と類似していた。しかし,至適反応温度は15℃であり,これは至適反応温度を高温領域にもつ哺乳類とは異なっていた。ニジマス白血球の15-LO酵素の最終精製段階における回収率は3.1%,精製純度が4.8倍と非常に不安定な酵素であることが明らかになった。", "subitem_description_type": "Other"}]}, "item_1_description_12": {"attribute_name": "抄録(英)", "attribute_value_mlt": [{"subitem_description": "15-Lipoxygenase activity with arachidonic acid as a substrate was detected in leukocyte extract from the kidney of rainbow trout (Oncorhynchus mykiss). 15-Lipoxygenase was purified by gel filtration followed by hydroxyapatite chromatography. The enzyme activity towards arachdonic acid was highest at pH 7.5 and at temperatures between 10℃ and 20℃. The molecular weight of enzyme estimated by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS-PAGE) was 70,000 near that of the enzyme from human leukocyte (65,000-75,000)[E.Sigal et.al : J. Biol. Chem., 263, 5328-5332(1988)].", "subitem_description_type": "Other"}]}, "item_1_full_name_7": {"attribute_name": "著者名よみ", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "40865", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "ヨシダ, ヨシオ"}]}, {"nameIdentifiers": [{"nameIdentifier": "40866", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "オキマス, エイジ"}]}, {"nameIdentifiers": [{"nameIdentifier": "40867", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "アメムラ, アキノリ"}]}]}, "item_1_full_name_8": {"attribute_name": "著者名(英)", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "40868", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Yoshida, Yoshio", "nameLang": "en"}]}, {"nameIdentifiers": [{"nameIdentifier": "40869", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Okimasu, Eiji", "nameLang": "en"}]}, {"nameIdentifiers": [{"nameIdentifier": "40870", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Amemura, Akinori", "nameLang": "en"}]}]}, "item_1_source_id_13": {"attribute_name": "雑誌書誌ID", "attribute_value_mlt": [{"subitem_source_identifier": "AN10409073", "subitem_source_identifier_type": "NCID"}]}, "item_1_text_10": {"attribute_name": "著者所属(英)", "attribute_value_mlt": [{"subitem_text_language": "en", "subitem_text_value": "Research Institute of Marine Bioresources, Fukuyama University"}, {"subitem_text_language": "en", "subitem_text_value": "Research Institute of Marine Bioresources, Fukuyama University"}, {"subitem_text_language": "en", "subitem_text_value": "Research Institute of Marine Bioresources, Fukuyama University"}]}, "item_1_text_2": {"attribute_name": "記事種別(日)", "attribute_value_mlt": [{"subitem_text_value": "報文"}]}, "item_1_text_3": {"attribute_name": "記事種別(英)", "attribute_value_mlt": [{"subitem_text_language": "en", "subitem_text_value": "Original Paper"}]}, "item_1_text_9": {"attribute_name": "著者所属(日)", "attribute_value_mlt": [{"subitem_text_value": "福山大学内海生物資源研究所:(現)共立商事(株)中央研究所"}, {"subitem_text_value": "福山大学内海生物資源研究所"}, {"subitem_text_value": "福山大学内海生物資源研究所"}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "1993-12-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "KJ00005783761.pdf", "filesize": [{"value": "1.0 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1000000.0, "url": {"url": "https://fukuyama-u.repo.nii.ac.jp/record/7431/files/KJ00005783761.pdf"}, "version_id": "5a5c0f90-57be-4f2a-8695-66a895fef0fd"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "ニジマス白血球のアラキドン酸15-リポキシゲナーゼの精製とその諸性質", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "ニジマス白血球のアラキドン酸15-リポキシゲナーゼの精製とその諸性質"}, {"subitem_title": "Purification and Characterization of Arachidonate 15-Lipoxygenase from Leukocytes of Rainbow Trout(Oncorhynchus mykiss)", "subitem_title_language": "en"}]}, "item_type_id": "1", "owner": "3", "path": ["657"], "permalink_uri": "https://fukuyama-u.repo.nii.ac.jp/records/7431", "pubdate": {"attribute_name": "公開日", "attribute_value": "1993-12-01"}, "publish_date": "1993-12-01", "publish_status": "0", "recid": "7431", "relation": {}, "relation_version_is_last": true, "title": ["ニジマス白血球のアラキドン酸15-リポキシゲナーゼの精製とその諸性質"], "weko_shared_id": -1}