WEKO3
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アミノ酸類の蛍光誘導体化試薬の開発及び生体試料中ヒドロキシプロリン、プロリン及びプロリルジペプチド類の高感度蛍光プレラベルHPLC分析
https://fukuyama-u.repo.nii.ac.jp/records/7102
https://fukuyama-u.repo.nii.ac.jp/records/7102bef2a552-21a1-46a0-9922-45da4a9e2d94
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00005784701.pdf (1.2 MB)
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| Item type | 紀要論文(ELS) / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 1999-01-01 | |||||
| タイトル | ||||||
| タイトル | アミノ酸類の蛍光誘導体化試薬の開発及び生体試料中ヒドロキシプロリン、プロリン及びプロリルジペプチド類の高感度蛍光プレラベルHPLC分析 | |||||
| タイトル | ||||||
| タイトル | Development of fluorescent labeling reagents for determination of amino acids and their applications for highly sensitive determination of hydroxyproline, proline and prolyl dipeptides in biological samples by pre-label HPLC with fluorescence detection | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| ページ属性 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | P(論文) | |||||
| 記事種別(日) | ||||||
| 値 | 総説 | |||||
| 著者名(日) |
井上, 裕文
× 井上, 裕文× 峠, 佳江× 鶴田, 泰人 |
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| 著者名よみ | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 37014 | |||||
| 姓名 | イノウエ, ヒロフミ | |||||
| 著者名よみ | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 37015 | |||||
| 姓名 | タオ, ヨシエ | |||||
| 著者名よみ | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 37016 | |||||
| 姓名 | ツルタ, ヤスト | |||||
| 著者名(英) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 37017 | |||||
| 姓名 | Inoue, Hirofumi | |||||
| 言語 | en | |||||
| 著者名(英) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 37018 | |||||
| 姓名 | Tao, Yoshie | |||||
| 言語 | en | |||||
| 著者名(英) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 37019 | |||||
| 姓名 | Tsuruta, Yasuto | |||||
| 言語 | en | |||||
| 著者所属(日) | ||||||
| 値 | 福山大学薬学部 | |||||
| 著者所属(日) | ||||||
| 値 | 福山大学薬学部 | |||||
| 著者所属(日) | ||||||
| 値 | 福山大学薬学部 | |||||
| 著者所属(英) | ||||||
| 言語 | en | |||||
| 値 | FACULTY OF PHARMACY & PHARMACEUTICAL SCIENCES FUKUYAMA UNIVERSITY | |||||
| 著者所属(英) | ||||||
| 言語 | en | |||||
| 値 | FACULTY OF PHARMACY & PHARMACEUTICAL SCIENCES FUKUYAMA UNIVERSITY | |||||
| 著者所属(英) | ||||||
| 言語 | en | |||||
| 値 | FACULTY OF PHARMACY & PHARMACEUTICAL SCIENCES FUKUYAMA UNIVERSITY | |||||
| 抄録(英) | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Highly sensitive fluorescent labeling reagents, 4-(5,6-dimethoxy-2-phthalimidinyl)phenylsulfonyl chloride (DPS-Cl) and 4-(5,6- dimethoxy-2-phthalimidinyl)-2-methoxyphenylsulfonyl chloride (DMS-Cl), for determination of amino acids by HPLC were developed. DPS-Cl and DMS-Cl reacted with amino acids in the basic medium to produce the corresponding fluorescent sulfonamides (DPS derivatives; Ex 315 nm, Em 385 nm: DMS derivatives; Ex 318 nm, Em 406 nm: in aqueous acetonitrile). When the reactivities of DPS-Cl and DMS-Cl were investigated by means of reversed phase HPLC using hydroxyproline (Hyp) and alanine (Ala) as model compounds, the reactions of Hyp with DPS-C1 and DMS-Cl were completed within 5 min at 25℃ and those of Ala were within 15 min at 70℃. The detection limits (signal-to-noise ratio = 3) of almost all amino acids labeled with DPS-Cl and DMS-Cl were less than 5 fmol/injection. DPS-Cl and DMS-Cl were applied to highly sensitive determination of Hyp, proline (Pro) and prolyl dipeptides in biological samples and HPLC methods for determination of free Hyp and Pro in serum, determination of total Hyp and Pro in serum and urine and determination of prolyl dipeptides, Pro and Hyp in urine were established. | |||||
| 雑誌書誌ID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN10064550 | |||||
| 書誌情報 |
福山大学薬学部研究年報 en : Annual report of the Faculty of Pharmacy & Pharmaceutical Sciences, Fukuyama University 巻 17, p. 1-25, 発行日 1999 |
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