{"created":"2023-06-19T09:51:37.200527+00:00","id":8839,"links":{},"metadata":{"_buckets":{"deposit":"1a26a077-e3b6-4a4c-8ad4-929e47e52042"},"_deposit":{"created_by":18,"id":"8839","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"8839"},"status":"published"},"_oai":{"id":"oai:fukuyama-u.repo.nii.ac.jp:00008839","sets":["502:503:627:764"]},"author_link":["45116","46005","45113","46256","46251","46257","46255","46252","46254","46253"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-12-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"29","bibliographicPageEnd":"48","bibliographicPageStart":"47","bibliographic_titles":[{"bibliographic_title":"福山大学薬学部研究年報"},{"bibliographic_title":"Annual report of the Faculty of Pharmacy & Pharmaceutical Sciences, Fukuyama University","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Lysosome-associated membrane protein-1 (LAMP-1) consists of a highly glycosylated luminal domain, a single-transmembrane domain and a short cytoplasmic tail that possesses a lysosome-targeting signal (GYQTI(382)) at the COOH terminus. It is hypothesized that the COOH-terminal isoleucine, I(382), could be substituted with any other bulky hydrophobic amino acid residue for LAMP-1 to exclusively localize in lysosomes. In order to test this hypothesis, we compared subcellular distribution of four substitution mutants with phenylalanine, leucine, methionine and valine at the COOH-terminus (termed I382F, I382L, I382M and I382V, respectively) with that of wild-type (WT)-LAMP-1. Double-labelled immunofluorescence analyses showed that these substitution mutants were localized as significantly to late endocytic organelles as WT-LAMP-1. However, the quantitative subcellular fractionation study revealed different distribution of WT-LAMP-1 and these four COOH-terminal mutants in late endosomes and dense secondary lysosomes. WT-LAMP-1 was accumulated three to six times more in the dense lysosomal fraction than the four mutants. The level of WT-LAMP-1 in late endosomal fraction was comparable to those of I382F, I382M and I382V. Conversely, I382L in the late endosomal fraction was approximately three times more abundant than WT-LAMP-1. These findings define the presence of isoleucine residue at the COOH-terminus of LAMP-1 as critical in governing its efficient delivery to secondary lysosomes and its ratio of lysosomes to late endosomes.","subitem_description_type":"Abstract"}]},"item_10002_description_6":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Lysosome-associated membrane protein-1 (LAMP-1) consists of a highly glycosylated luminal domain, a single-transmembrane domain and a short cytoplasmic tail that possesses a lysosome-targeting signal (GYQTI(382)) at the COOH terminus. It is hypothesized that the COOH-terminal isoleucine, I(382), could be substituted with any other bulky hydrophobic amino acid residue for LAMP-1 to exclusively localize in lysosomes. In order to test this hypothesis, we compared subcellular distribution of four substitution mutants with phenylalanine, leucine, methionine and valine at the COOH-terminus (termed I382F, I382L, I382M and I382V, respectively) with that of wild-type (WT)-LAMP-1. Double-labelled immunofluorescence analyses showed that these substitution mutants were localized as significantly to late endocytic organelles as WT-LAMP-1. However, the quantitative subcellular fractionation study revealed different distribution of WT-LAMP-1 and these four COOH-terminal mutants in late endosomes and dense secondary lysosomes. WT-LAMP-1 was accumulated three to six times more in the dense lysosomal fraction than the four mutants. The level of WT-LAMP-1 in late endosomal fraction was comparable to those of I382F, I382M and I382V. Conversely, I382L in the late endosomal fraction was approximately three times more abundant than WT-LAMP-1. These findings define the presence of isoleucine residue at the COOH-terminus of LAMP-1 as critical in governing its efficient delivery to secondary lysosomes and its ratio of lysosomes to late endosomes.","subitem_description_type":"Other"}]},"item_10002_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"45116","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000005185543","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000005185543"}],"names":[{"name":"赤崎, 健司"}]},{"nameIdentifiers":[{"nameIdentifier":"46256","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000337100380","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000337100380"}],"names":[{"name":"末延, 道尚"}]},{"nameIdentifiers":[{"nameIdentifier":"46257","nameIdentifierScheme":"WEKO"}],"names":[{"name":"向田, 真希"}]},{"nameIdentifiers":[{"nameIdentifier":"45113","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000014625611","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000014625611"}],"names":[{"name":"道原, 明宏"}]},{"nameIdentifiers":[{"nameIdentifier":"46005","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000040182969","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000040182969"}],"names":[{"name":"和田, 郁夫"}]}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"福山大学薬学部"}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN10064550","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0288-724X","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Akasaki, Kenji"}],"nameIdentifiers":[{"nameIdentifier":"46251","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Suenobu, Michihisa"}],"nameIdentifiers":[{"nameIdentifier":"46252","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mukaida, Maki"}],"nameIdentifiers":[{"nameIdentifier":"46253","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Michihara, Akihiro"}],"nameIdentifiers":[{"nameIdentifier":"46254","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Wada, Ikuo"}],"nameIdentifiers":[{"nameIdentifier":"46255","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-12-27"}],"displaytype":"detail","filename":"リソソーム膜タンパク質LAMP-1のC 末端アミノ酸残基のイソロイシンが効率的なリソソームへの輸送に最適である.pdf","filesize":[{"value":"615.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"赤﨑健司(発表論文抄録(2010))","url":"https://fukuyama-u.repo.nii.ac.jp/record/8839/files/リソソーム膜タンパク質LAMP-1のC 末端アミノ酸残基のイソロイシンが効率的なリソソームへの輸送に最適である.pdf"},"version_id":"ff58fd64-d586-4ac9-9e5d-d1b183b4134e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Amino Acid Motifs","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Amino Acid Substitution","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Animals","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell Fractionation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Endosomes","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Haplorhini","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Isoleucine","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Lysosomal-Associated Membrane Protein 1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Lysosomes","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Microscopy, Fluorescence","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Mutagenesis, Site-Directed","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Protein Structure, Tertiary","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Protein Transport","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Transfection","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Tumor Cells, Cultured","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"リソソーム膜タンパク質LAMP-1のC 末端アミノ酸残基のイソロイシンが効率的なリソソームへの輸送に最適である(発表論文抄録(2010))","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"リソソーム膜タンパク質LAMP-1のC 末端アミノ酸残基のイソロイシンが効率的なリソソームへの輸送に最適である(発表論文抄録(2010))"},{"subitem_title":"COOH-terminal isoleucine of lysosome-associated membrane protein-1 is optimal for its efficient targeting to dense secondary lysosomes.","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"18","path":["764"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-12-27"},"publish_date":"2017-12-27","publish_status":"0","recid":"8839","relation_version_is_last":true,"title":["リソソーム膜タンパク質LAMP-1のC 末端アミノ酸残基のイソロイシンが効率的なリソソームへの輸送に最適である(発表論文抄録(2010))"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-06-19T10:15:40.927012+00:00"}