{"created":"2023-06-19T09:51:34.444744+00:00","id":8776,"links":{},"metadata":{"_buckets":{"deposit":"5f30bfd6-33d3-4cff-9ddd-547ea9b73e08"},"_deposit":{"created_by":18,"id":"8776","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"8776"},"status":"published"},"_oai":{"id":"oai:fukuyama-u.repo.nii.ac.jp:00008776","sets":["502:503:627:783"]},"author_link":["45914","45910","45911","45912","45915","45913"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-12-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"31","bibliographicPageEnd":"43","bibliographicPageStart":"43","bibliographic_titles":[{"bibliographic_title":"福山大学薬学部研究年報"},{"bibliographic_title":"Annual report of the Faculty of Pharmacy & Pharmaceutical Sciences, Fukuyama University","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"13C標識テオフィリン(TP)経口投与後のヒト尿中代謝物の13C-NMRによる定量を検討した。腎及び肝機能正常な5人の男性被験者に13C標識テオフィリン(1,7-ジメチル-13C2-キサンチン)50mgを投与し,Sep-Pac Vac RCカラムにより固相抽出した。抽出試料より未変化TP及び1,3-ジメチル尿酸(13U),3-メチルキサンチン(3X),1-メチル尿酸(1U)をNMRにより特異的に検出できた。13C標識代謝物標品と内部標準として[3-メチル-13C]カフェイン(3-13C-CF)のシグナル積算値の比はそれらのモル比に比例した。他の代謝物についても同様の関係が成立するものと推定される。13C標識TPの尿中代謝物の定量結果より,喫煙者と非喫煙者の間には肝臓のCYP1A2活性を反映した著しい個人差が認められた。薬物代謝研究において,13C-NMR法は代謝物の検索及び定量に有用であることが認められた。","subitem_description_type":"Abstract"}]},"item_10002_description_6":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Determination of human urinary metabolites after oral administration of 13C-labeled theophylline (TP) was examined by 13C-NMR spectroscopy. 13C-labeled TP(1,7-dimethyl-13C2-xanthine)was administered at a dose of 50mg to 5male volunteers who have normal hepatic and renal functions. Urinary samples were prepared by solid phase extraction with Sep-Pac Vac RC columns. NMR spectra of the urinary extracts indicated the specific resonances signals of unchanged TP and its main metabolites, 1, 3-dimethyluric acid(13U), 3-methylxanthine(3X) and 1-methyluric acid(1U). The integral signal intensity ratio of authentic 13C-labeled metabolites to [3-methyl-13C] caffeine(3-13C-CF) as an internal standard was directly proportional to the molar ratio of the compounds. Similar relationship was expected to other metabolites. From the results of quantification of urinary metabolites of 13C-labeled TP, remarkable individual difference was observed in the urinary excretion of metabolites between smoker and nonsmoker due to influence of hepatic CYP1A2 activity. In drug metabolism study, 13C-NMR spectroscopy with 13C-tracer was useful technique for detection and quantification of the metabolites.","subitem_description_type":"Other"}]},"item_10002_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"45913","nameIdentifierScheme":"WEKO"}],"names":[{"name":"GOROMARU, Tsuyoshi"}]},{"nameIdentifiers":[{"nameIdentifier":"45914","nameIdentifierScheme":"WEKO"}],"names":[{"name":"KAKIHARA, Yoshie"}]},{"nameIdentifiers":[{"nameIdentifier":"45915","nameIdentifierScheme":"WEKO"}],"names":[{"name":"MOTOYASHIKI, Toshio"}]}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"福山大学薬学部"}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN10064550","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0288-724X","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"五郎丸, 毅"}],"nameIdentifiers":[{"nameIdentifier":"45910","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"柿原, 良枝"}],"nameIdentifiers":[{"nameIdentifier":"45911","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"本屋敷, 敏雄"}],"nameIdentifiers":[{"nameIdentifier":"45912","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-12-20"}],"displaytype":"detail","filename":"31-43.pdf","filesize":[{"value":"135.7 kB"}],"format":"application/pdf","license_note":"Copyright (c) 2013 by Fukuyama University","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"五郎丸毅(発表論文抄録(2012))","url":"https://fukuyama-u.repo.nii.ac.jp/record/8776/files/31-43.pdf"},"version_id":"87570c88-90f4-4afc-a6ef-712eec6bcb7f"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"determination","subitem_subject_scheme":"Other"},{"subitem_subject":"urinary metabolites","subitem_subject_scheme":"Other"},{"subitem_subject":"¹³C-labeledtheophylline","subitem_subject_scheme":"Other"},{"subitem_subject":"NMR","subitem_subject_scheme":"Other"},{"subitem_subject":"determination","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"urinary metabolites","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"¹³C-labeledtheophylline","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"NMR","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"^<13>C標識テオフィリン服用後のヒト尿中代謝物のNMRによる定量(発表論文抄録(2012))","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"^<13>C標識テオフィリン服用後のヒト尿中代謝物のNMRによる定量(発表論文抄録(2012))"},{"subitem_title":"Determination of Human Urinary Metabolites by NMR after Administration of ^<13>C-labeled Theophylline","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"18","path":["783"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-12-20"},"publish_date":"2017-12-20","publish_status":"0","recid":"8776","relation_version_is_last":true,"title":["^<13>C標識テオフィリン服用後のヒト尿中代謝物のNMRによる定量(発表論文抄録(2012))"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-06-19T10:17:10.893043+00:00"}