{"created":"2023-06-19T09:51:34.222043+00:00","id":8771,"links":{},"metadata":{"_buckets":{"deposit":"f7b3877e-feee-4911-a581-ed732e2beda1"},"_deposit":{"created_by":18,"id":"8771","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"8771"},"status":"published"},"_oai":{"id":"oai:fukuyama-u.repo.nii.ac.jp:00008771","sets":["502:503:627:783"]},"author_link":["45845","45848","45849","45853","45850","45844","45842","45648","45854","45852","45851","45846","45843","45847"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-12-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"31","bibliographicPageEnd":"35","bibliographicPageStart":"35","bibliographic_titles":[{"bibliographic_title":"福山大学薬学部研究年報"},{"bibliographic_title":"Protein and peptide letters","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Measles virus (MV), one of the most contagious agents, infects immune cells using the signaling lymphocyte activation molecule (SLAM) on the cell surface. A complex of SLAM and the attachment protein, hemagglutinin (MVH), has remained elusive due to the intrinsic handling difficulty including glycosylation. Furthermore, crystals obtained of this complex are either nondiffracting or poorly-diffracting. To solve this problem, we designed a systematic approach using a combination of the following techniques; (1) a transient expression system in HEK293SGnTI(-) cells, (2) lysine methylation, (3) structure-guided mutagenesis directed at better crystal packing, (4) Endo H treatment, (5) single-chain formation for stable complex, and (6) floating-drop vapor diffusion. Using our approach, the receptor-binding head domain of MV-H covalently fused with SLAM was successfully crystallized and diffraction was improved from 4.5 Å to a final resolution of 3.15 Å . These combinational methods would be useful as crystallization strategies for complexes of glycoproteins and their receptors.","subitem_description_type":"Abstract"}]},"item_10002_description_6":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Measles virus (MV), one of the most contagious agents, infects immune cells using the signaling lymphocyte activation molecule (SLAM) on the cell surface. A complex of SLAM and the attachment protein, hemagglutinin (MVH), has remained elusive due to the intrinsic handling difficulty including glycosylation. Furthermore, crystals obtained of this complex are either nondiffracting or poorly-diffracting. To solve this problem, we designed a systematic approach using a combination of the following techniques; (1) a transient expression system in HEK293SGnTI(-) cells, (2) lysine methylation, (3) structure-guided mutagenesis directed at better crystal packing, (4) Endo H treatment, (5) single-chain formation for stable complex, and (6) floating-drop vapor diffusion. Using our approach, the receptor-binding head domain of MV-H covalently fused with SLAM was successfully crystallized and diffraction was improved from 4.5 Å to a final resolution of 3.15 Å . These combinational methods would be useful as crystallization strategies for complexes of glycoproteins and their receptors.","subitem_description_type":"Other"}]},"item_10002_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"45849","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000014655264","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000014655264"}],"names":[{"name":"橋口, 隆生"}]},{"nameIdentifiers":[{"nameIdentifier":"45850","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000002741954","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000002741954"}],"names":[{"name":"尾瀬, 農之"}]},{"nameIdentifiers":[{"nameIdentifier":"45851","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000344119323","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000344119323"}],"names":[{"name":"久保田, 万理恵"}]},{"nameIdentifiers":[{"nameIdentifier":"45852","nameIdentifierScheme":"WEKO"}],"names":[{"name":"真板, 宣夫"}]},{"nameIdentifiers":[{"nameIdentifier":"45648","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000279914332","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000279914332"}],"names":[{"name":"上敷領, 淳"}]},{"nameIdentifiers":[{"nameIdentifier":"45853","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000010322752","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000010322752"}],"names":[{"name":"前仲, 勝実"}]},{"nameIdentifiers":[{"nameIdentifier":"45854","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000040182365","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000040182365"}],"names":[{"name":"柳, 雄介"}]}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"福山大学薬学部"}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN10064550","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0288-724X","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hashiguchi, Takao"}],"nameIdentifiers":[{"nameIdentifier":"45842","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ose, Toyoyuki"}],"nameIdentifiers":[{"nameIdentifier":"45843","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kubota, Marie"}],"nameIdentifiers":[{"nameIdentifier":"45844","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Maita, Nobuo"}],"nameIdentifiers":[{"nameIdentifier":"45845","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kamishikiryo, Jun"}],"nameIdentifiers":[{"nameIdentifier":"45846","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Maenaka, Katsumi"}],"nameIdentifiers":[{"nameIdentifier":"45847","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yanagi, Yusuke"}],"nameIdentifiers":[{"nameIdentifier":"45848","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-12-20"}],"displaytype":"detail","filename":"31-35.pdf","filesize":[{"value":"137.6 kB"}],"format":"application/pdf","license_note":"Copyright (c) 2013 by Fukuyama University","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"橋口隆生(発表論文抄録(2012))","url":"https://fukuyama-u.repo.nii.ac.jp/record/8771/files/31-35.pdf"},"version_id":"5f40da91-d993-4155-b2f0-d94f2999e3eb"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Antigens, CD","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Crystallization","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Glycoproteins","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"HEK293 Cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Hemagglutinins","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Humans","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Macromolecular Substances","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Measles virus","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Methylation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Receptors, Cell Surface","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Signaling Lymphocytic Activation Molecule Family Member 1","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"糖たんぱく質-受容体複合体の結晶化戦略 : 麻疹ウイルスヘマグルチニンとその細胞受容体SLAM複合体の結晶構造解析(発表論文抄録(2012))","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"糖たんぱく質-受容体複合体の結晶化戦略 : 麻疹ウイルスヘマグルチニンとその細胞受容体SLAM複合体の結晶構造解析(発表論文抄録(2012))"},{"subitem_title":"Crystallization strategy for the glycoprotein-receptor complex between measles virus hemagglutinin and its cellular receptor SLAM.","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"18","path":["783"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-12-20"},"publish_date":"2017-12-20","publish_status":"0","recid":"8771","relation_version_is_last":true,"title":["糖たんぱく質-受容体複合体の結晶化戦略 : 麻疹ウイルスヘマグルチニンとその細胞受容体SLAM複合体の結晶構造解析(発表論文抄録(2012))"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-06-19T10:17:17.586770+00:00"}