{"created":"2023-06-19T09:51:26.716223+00:00","id":8612,"links":{},"metadata":{"_buckets":{"deposit":"f8addb14-ded2-4094-8d4c-4e7242871e89"},"_deposit":{"created_by":18,"id":"8612","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"8612"},"status":"published"},"_oai":{"id":"oai:fukuyama-u.repo.nii.ac.jp:00008612","sets":["502:503:627:763"]},"author_link":["45374","45376","45375"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"28","bibliographicPageEnd":"65","bibliographicPageStart":"65","bibliographic_titles":[{"bibliographic_title":"福山大学薬学部研究年報"},{"bibliographic_title":"Annual report of the Faculty of Pharmacy & Pharmaceutical Sciences, Fukuyama University","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Retinoic acid (RA) induces F9 cells, the mouse teratocarcinoma cells, to differentiate into primitive endoderm and further into visceral and parietal endoderm depending on the culture conditions. To elucidate the instructive mechanisms involved in the differentiation steps we investigated the effects of Wnt-signaling members, Wnt3a and beta-catenin, on the differentiation of F9 cells and beta-catenin-deficient F9 cells (betaT cells). RA up-regulated the expression of differentiation markers for primitive, visceral and parietal endoderm in F9 cells but not for visceral endoderm in betaT cells. Wnt3a or leukemia inhibitory factor (LIF) inhibited the RA-induced differentiation in F9 cells. LIF but not Wnt3a could inhibit differentiation in betaT cells. RA evoked ZO-1alpha+ signals at cell-to-cell contacts in F9 cells in a Wnt3a sensitive manner. The results suggest that Wnt3a inhibits differentiation into endoderm through a pathway involving beta-catenin, and beta-catenin might be necessary in the process leading from primitive to visceral endoderm in F9 cells.","subitem_description_type":"Abstract"}]},"item_10002_description_6":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Retinoic acid (RA) induces F9 cells, the mouse teratocarcinoma cells, to differentiate into primitive endoderm and further into visceral and parietal endoderm depending on the culture conditions. To elucidate the instructive mechanisms involved in the differentiation steps we investigated the effects of Wnt-signaling members, Wnt3a and beta-catenin, on the differentiation of F9 cells and beta-catenin-deficient F9 cells (betaT cells). RA up-regulated the expression of differentiation markers for primitive, visceral and parietal endoderm in F9 cells but not for visceral endoderm in betaT cells. Wnt3a or leukemia inhibitory factor (LIF) inhibited the RA-induced differentiation in F9 cells. LIF but not Wnt3a could inhibit differentiation in betaT cells. RA evoked ZO-1alpha+ signals at cell-to-cell contacts in F9 cells in a Wnt3a sensitive manner. The results suggest that Wnt3a inhibits differentiation into endoderm through a pathway involving beta-catenin, and beta-catenin might be necessary in the process leading from primitive to visceral endoderm in F9 cells.","subitem_description_type":"Other"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"福山大学薬学部"}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN10064550","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0288-724X","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"井上, 敦子"}],"nameIdentifiers":[{"nameIdentifier":"45374","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000005169953","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000005169953"}]},{"creatorNames":[{"creatorName":"永渕, 昭良"}],"nameIdentifiers":[{"nameIdentifier":"45375","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000080218023","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000080218023"}]},{"creatorNames":[{"creatorName":"菊池, 章"}],"nameIdentifiers":[{"nameIdentifier":"45376","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000002745536","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000002745536"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-01-30"}],"displaytype":"detail","filename":"レチノイン酸によるF ９細胞のWnt 依存的分化について.pdf","filesize":[{"value":"519.3 kB"}],"format":"application/pdf","license_note":"Copyright (c) 2010 by Fukuyama University","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"レチノイン酸によるF9細胞のWnt依存的分化について","url":"https://fukuyama-u.repo.nii.ac.jp/record/8612/files/レチノイン酸によるF ９細胞のWnt 依存的分化について.pdf"},"version_id":"67b2e03b-68af-40a0-83dd-2bb9c6d4e02b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Animals","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell Differentiation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell Line, Tumor","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Endoderm","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Gene Expression","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Leukemia Inhibitory Factor","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Membrane Proteins","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Mice","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Phosphoproteins","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Tretinoin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Wnt Proteins","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Wnt3 Protein","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Wnt3A Protein","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Zonula Occludens-1 Protein","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"beta Catenin","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"レチノイン酸によるF9細胞のWnt依存的分化について(発表論文抄録(2009))","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"レチノイン酸によるF9細胞のWnt依存的分化について(発表論文抄録(2009))"},{"subitem_title":"Retinoic acid induces discrete Wnt-signaling-dependent differentiation in F9 cells.","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"18","path":["763"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-01-30"},"publish_date":"2017-01-30","publish_status":"0","recid":"8612","relation_version_is_last":true,"title":["レチノイン酸によるF9細胞のWnt依存的分化について(発表論文抄録(2009))"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-06-19T10:20:31.989971+00:00"}