@article{oai:fukuyama-u.repo.nii.ac.jp:00008569,
 author = {鶴田, 泰人 and 松本, 真弥 and 井上, 裕文 and 宗村, 小夜香 and 石津, 隆 and 山野, 茂 and 井口, 東郎},
 issue = {27},
 journal = {福山大学薬学部研究年報, Annual report of the Faculty of Pharmacy & Pharmaceutical Sciences, Fukuyama University},
 month = {Dec},
 note = {A simple and highly sensitive HPLC for the determination of <i>N</i>-ethylglycine in urine was developed.  The labeling reaction of <i>N</i>-ethylglycine with 4-(5,6-dimethoxy-2-phthalimidinyl)-2-methoxyphenylsulfonyl chloride was carried out at 70°C for 15 min at pH 9.0.  The fluorescent derivative was separated on a reversed-phase column and detected at excitation and emission wavelengths of 320 and 400 nm, respectively.  The detection limit of <i>N</i>-ethylglycine was 15 fmol (<i>S/N</i> = 3).  The recovery of <i>N</i>-ethylglycine added to urine was 101.9%.  The concentration of <i>N</i>-ethylglycine in urine of cancer patients with metastatic bone disease was 11.3 ± 22.0 nmol/mg creatinine, and that of normal subject was 0.4 ± 0.4 nmol/mg creatinine., A simple and highly sensitive HPLC for the determination of <i>N</i>-ethylglycine in urine was developed.  The labeling reaction of <i>N</i>-ethylglycine with 4-(5,6-dimethoxy-2-phthalimidinyl)-2-methoxyphenylsulfonyl chloride was carried out at 70°C for 15 min at pH 9.0.  The fluorescent derivative was separated on a reversed-phase column and detected at excitation and emission wavelengths of 320 and 400 nm, respectively.  The detection limit of <i>N</i>-ethylglycine was 15 fmol (<i>S/N</i> = 3).  The recovery of <i>N</i>-ethylglycine added to urine was 101.9%.  The concentration of <i>N</i>-ethylglycine in urine of cancer patients with metastatic bone disease was 11.3 ± 22.0 nmol/mg creatinine, and that of normal subject was 0.4 ± 0.4 nmol/mg creatinine.},
 pages = {37--37},
 title = {蛍光誘導体化試薬4-(5，6-ジメトキシ-2-フタルイミジニル)-2-メトキシフェニルスルホニルクロリドを用いる転移性骨腫瘍患者の尿中N-エチルグリシンのHPLCによる定量(発表論文抄録(2008))},
 year = {2009}
}