{"created":"2023-06-19T09:51:22.458383+00:00","id":8541,"links":{},"metadata":{"_buckets":{"deposit":"91eaf8be-ee74-4c8d-bcca-8a81bb00c335"},"_deposit":{"created_by":18,"id":"8541","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"8541"},"status":"published"},"_oai":{"id":"oai:fukuyama-u.repo.nii.ac.jp:00008541","sets":["502:503:627:761"]},"author_link":["45121","45120","45123","45119","45118","45122"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-12-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"26","bibliographicPageEnd":"58","bibliographicPageStart":"57","bibliographic_titles":[{"bibliographic_title":"福山大学薬学部研究年報"},{"bibliographic_title":"Annual report of the Faculty of Pharmacy & Pharmaceutical Sciences, Fukuyama University","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The previous studies from our laboratory reported that benzo(a)pyrene (Bap) influenced efflux transport of rhodamine 123 (Rho-123) by induction of P-glycoprotein (P-gp) in Caco-2 cells. The present study investigated whether induction of P-gp and the enhanced efflux transport of Rho-123 were caused by benzo(e)pyrene (Bep), which has a structure similar to Bap, but is not a carcinogenic compound. In Caco-2 monolayer exposed to 50 microM Bep for 72 h, the ratio of the apparent permeability coefficient (P(app)) of Rho-123 efflux increased significantly compared to that of the control monolayer. Similarly, a significant increase in expression of MDR1 mRNA and of P-gp at the protein level were detected by RT-PCR and by Western blot analysis, respectively, in Caco-2 cells exposed to Bep, compared to that of the control. Caco-2 cells exposed to Bep showed oxidative stress that was detected by fluorescence microscopy using aminophenyl fluorescein. However, the oxidative stress was weaker compared with that of Bap. The cellular GSH content was decreased to 80% or 59% of control cells, respectively, in Caco-2 cells exposed to either Bep or Bap. Our results further show that Bep or Bap-induced P-gp in Caco-2 cells might have been the result of oxidative stress rather than DNA damage.","subitem_description_type":"Abstract"}]},"item_10002_description_6":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"The previous studies from our laboratory reported that benzo(a)pyrene (Bap) influenced efflux transport of rhodamine 123 (Rho-123) by induction of P-glycoprotein (P-gp) in Caco-2 cells. The present study investigated whether induction of P-gp and the enhanced efflux transport of Rho-123 were caused by benzo(e)pyrene (Bep), which has a structure similar to Bap, but is not a carcinogenic compound. In Caco-2 monolayer exposed to 50 microM Bep for 72 h, the ratio of the apparent permeability coefficient (P(app)) of Rho-123 efflux increased significantly compared to that of the control monolayer. Similarly, a significant increase in expression of MDR1 mRNA and of P-gp at the protein level were detected by RT-PCR and by Western blot analysis, respectively, in Caco-2 cells exposed to Bep, compared to that of the control. Caco-2 cells exposed to Bep showed oxidative stress that was detected by fluorescence microscopy using aminophenyl fluorescein. However, the oxidative stress was weaker compared with that of Bap. The cellular GSH content was decreased to 80% or 59% of control cells, respectively, in Caco-2 cells exposed to either Bep or Bap. Our results further show that Bep or Bap-induced P-gp in Caco-2 cells might have been the result of oxidative stress rather than DNA damage.","subitem_description_type":"Other"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"福山大学薬学部"}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN10064550","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0288-724X","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"杉原, 成美"}],"nameIdentifiers":[{"nameIdentifier":"45118","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000005187246","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000005187246"}]},{"creatorNames":[{"creatorName":"戸山, 久美子"}],"nameIdentifiers":[{"nameIdentifier":"45119","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000014625974","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000014625974"}]},{"creatorNames":[{"creatorName":"岡本, 達明"}],"nameIdentifiers":[{"nameIdentifier":"45120","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"門脇, 正明"}],"nameIdentifiers":[{"nameIdentifier":"45121","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000014625941","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000014625941"}]},{"creatorNames":[{"creatorName":"寺尾, 和美"}],"nameIdentifiers":[{"nameIdentifier":"45122","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"古野, 浩二"}],"nameIdentifiers":[{"nameIdentifier":"45123","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000005185545","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000005185545"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-11-30"}],"displaytype":"detail","filename":"57 単層培養したCaco-2 .pdf","filesize":[{"value":"183.6 kB"}],"format":"application/pdf","license_note":"Copyright (c) 2008 by Fukuyama University","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"単層培養したCaco-2 細胞におけるP- 糖タンパク質関与の輸送に及ぼすベンゾ[e] ピレンとベンゾ[a] ピレンの影響：比較試験","url":"https://fukuyama-u.repo.nii.ac.jp/record/8541/files/57 単層培養したCaco-2 .pdf"},"version_id":"4e11231f-6f16-46fa-ad2a-3cfb9cb276ca"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Benzo(a)pyrene","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Benzopyrenes","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Biological Transport, Active","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Blotting, Western","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Caco-2 Cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Carcinogens","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell Survival","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA Damage","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Glutathione","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Humans","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Microscopy, Fluorescence","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Oxidative Stress","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"P-Glycoprotein","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Polycyclic Hydrocarbons, Aromatic","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"RNA, Messenger","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Reverse Transcriptase Polymerase Chain Reaction","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Rhodamine 123","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"単層培養したCaco-2 細胞におけるP- 糖タンパク質関与の輸送に及ぼすベンゾ[e] ピレンとベンゾ[a] ピレンの影響：比較試験(発表論文抄録(2007))","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"単層培養したCaco-2 細胞におけるP- 糖タンパク質関与の輸送に及ぼすベンゾ[e] ピレンとベンゾ[a] ピレンの影響：比較試験(発表論文抄録(2007))"},{"subitem_title":"Effects of benzo(e)pyrene and benzo(a)pyrene on P-glycoprotein-mediated transport in Caco-2 cell monolayer: a comparative approach.","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"18","path":["761"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-11-30"},"publish_date":"2016-11-30","publish_status":"0","recid":"8541","relation_version_is_last":true,"title":["単層培養したCaco-2 細胞におけるP- 糖タンパク質関与の輸送に及ぼすベンゾ[e] ピレンとベンゾ[a] ピレンの影響：比較試験(発表論文抄録(2007))"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-06-19T10:21:55.606693+00:00"}