{"created":"2023-06-19T09:51:22.395327+00:00","id":8540,"links":{},"metadata":{"_buckets":{"deposit":"b091cb41-0e15-4497-b832-494060fb0578"},"_deposit":{"created_by":18,"id":"8540","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"8540"},"status":"published"},"_oai":{"id":"oai:fukuyama-u.repo.nii.ac.jp:00008540","sets":["502:503:627:761"]},"author_link":["45116","45113","45117","45115","45114"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-12-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"26","bibliographicPageEnd":"56","bibliographicPageStart":"56","bibliographic_titles":[{"bibliographic_title":"福山大学薬学部研究年報"},{"bibliographic_title":"Annual report of the Faculty of Pharmacy & Pharmaceutical Sciences, Fukuyama University","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We examined the change in the subcellular distribution of a lysosomal enzyme, beta-glucuronidase (beta-G), caused by decreased cholesterol levels in mouse melanoma cells using an HMG-CoA reductase inhibitor, lovastatin and lipoprotein-deficient serum (LDS). There was a decrease in the cholesterol content of the cells and increased secretion of the mature form of beta-G located in lysosomes, as documented by Percoll density gradient fractionation, digitonin permeabilization and immunoprecipitation. Furthermore, another lysosomal enzyme, cathepsin H, was found to be released in the medium from cells treated with lovastatin. Both the precursor and mature forms of cathepsin H were detected in the medium of treated cells. Next, when cells were treated with LDS without lovastatin, concomitantly with the decrease in the levels of cholesterol and beta-G activity in the cells, beta-G activity in the medium increased. Also, the ratio of beta-G (3.2-fold) released in the medium from cells treated with Dulbecco's modified Eagle medium (D-MEM) containing lovastatin and LDS was higher than that (2.3-fold) on treatment with D-MEM containing LDS without lovastatin. From these results, it was suggested that the exocytosis of mature enzymes from lysosomes into the medium or mis-sorting of the lysosomal precursor forms to the medium was caused by the lovastatin- and/or LDS-induced decrease in the cholesterol content of the cells, although the mechanism of secretion by lysosomal enzymes differed somewhat.","subitem_description_type":"Abstract"}]},"item_10002_description_6":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"We examined the change in the subcellular distribution of a lysosomal enzyme, beta-glucuronidase (beta-G), caused by decreased cholesterol levels in mouse melanoma cells using an HMG-CoA reductase inhibitor, lovastatin and lipoprotein-deficient serum (LDS). There was a decrease in the cholesterol content of the cells and increased secretion of the mature form of beta-G located in lysosomes, as documented by Percoll density gradient fractionation, digitonin permeabilization and immunoprecipitation. Furthermore, another lysosomal enzyme, cathepsin H, was found to be released in the medium from cells treated with lovastatin. Both the precursor and mature forms of cathepsin H were detected in the medium of treated cells. Next, when cells were treated with LDS without lovastatin, concomitantly with the decrease in the levels of cholesterol and beta-G activity in the cells, beta-G activity in the medium increased. Also, the ratio of beta-G (3.2-fold) released in the medium from cells treated with Dulbecco's modified Eagle medium (D-MEM) containing lovastatin and LDS was higher than that (2.3-fold) on treatment with D-MEM containing LDS without lovastatin. From these results, it was suggested that the exocytosis of mature enzymes from lysosomes into the medium or mis-sorting of the lysosomal precursor forms to the medium was caused by the lovastatin- and/or LDS-induced decrease in the cholesterol content of the cells, although the mechanism of secretion by lysosomal enzymes differed somewhat.","subitem_description_type":"Other"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"福山大学薬学部"}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN10064550","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0288-724X","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"道原, 明宏"}],"nameIdentifiers":[{"nameIdentifier":"45113","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000014625611","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000014625611"}]},{"creatorNames":[{"creatorName":"戸田, 憲"}],"nameIdentifiers":[{"nameIdentifier":"45114","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000014625939 ","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000014625939 "}]},{"creatorNames":[{"creatorName":"末延, 道尚"}],"nameIdentifiers":[{"nameIdentifier":"45115","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"赤崎, 健司"}],"nameIdentifiers":[{"nameIdentifier":"45116","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000005185543","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000005185543"}]},{"creatorNames":[{"creatorName":"辻, 宏"}],"nameIdentifiers":[{"nameIdentifier":"45117","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000005185546","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000005185546"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-11-30"}],"displaytype":"detail","filename":"56 マウスメラノーマ中のコレステロール.pdf","filesize":[{"value":"120.3 kB"}],"format":"application/pdf","license_note":"Copyright (c) 2008 by Fukuyama University","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"マウスメラノーマ中のコレステロール低下はリソソーム酵素の分泌を引き起こす","url":"https://fukuyama-u.repo.nii.ac.jp/record/8540/files/56 マウスメラノーマ中のコレステロール.pdf"},"version_id":"982e866f-146b-4f0c-95b3-30429e3ea744"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Animals","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Anticholesteremic Agents","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cathepsin H","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cathepsins","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell Line, Tumor","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell Survival","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cholesterol","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Culture Media","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cysteine Endopeptidases","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cytochrome P-450 CYP2B1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Endoplasmic Reticulum","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Glucuronidase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Kinetics","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Lipoproteins","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Lovastatin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Lysosomes","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Male","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Melanoma, Experimental","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Mice","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Rats","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Rats, Wistar","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"マウスメラノーマ中のコレステロール低下はリソソーム酵素の分泌を引き起こす(発表論文抄録(2007))","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"マウスメラノーマ中のコレステロール低下はリソソーム酵素の分泌を引き起こす(発表論文抄録(2007))"},{"subitem_title":"Decrease of cholesterol in mouse melanoma causes secretion of lysosomal enzymes.","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"18","path":["761"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-11-30"},"publish_date":"2016-11-30","publish_status":"0","recid":"8540","relation_version_is_last":true,"title":["マウスメラノーマ中のコレステロール低下はリソソーム酵素の分泌を引き起こす(発表論文抄録(2007))"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-06-19T10:21:56.778032+00:00"}