{"created":"2023-06-19T09:51:02.006036+00:00","id":8020,"links":{},"metadata":{"_buckets":{"deposit":"e9b43315-cf83-4959-9008-d94442ace49e"},"_deposit":{"created_by":3,"id":"8020","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"8020"},"status":"published"},"_oai":{"id":"oai:fukuyama-u.repo.nii.ac.jp:00008020","sets":["502:505:675:704"]},"author_link":["43018","43016","43015","43017"],"item_1_alternative_title_5":{"attribute_name":"論文名よみ","attribute_value_mlt":[{"subitem_alternative_title":"Purification and Characterization of α-Galactosidase from the Yeast Trichosporon cutaneum JCM 2947"}]},"item_1_biblio_info_14":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1997-12","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"71","bibliographicPageStart":"67","bibliographicVolumeNumber":"21","bibliographic_titles":[{"bibliographic_title":"福山大学工学部紀要"}]}]},"item_1_description_1":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_1_description_12":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"α-Galactosidase was purified to homogeneity from the culture fluid of the yeast Trichosporon cutaneum JCM 2947 (=T. beigelii CBS 5790) by column chromatography on DEAE-Toyopearl 650M, Butyl-Toyopearl 650M, hydroxylapatite and Sephacryl S-200. The molecular weight of the purified enzyme was estimated to be 85,700 by SDS-polyacrylamide gel electrophoresis and 321,000 by gel filtration. Optimum pH and temperature were 5.0 to 6.0 and 45℃, respectively. The K_m (mM) for p-, o-, m-nitrophenyl α-D-galactopyranoside, melibiose, raffinose, and stachyose were 0.14, 0.25, 0.49, 6.0, 48, and 68, respectively, and V_<max>. (μmol/min/mg protein) for those substrates were 66.2, 22.6, 12.6, 12.6, 28.9, and 18.0, respectively.","subitem_description_type":"Other"}]},"item_1_full_name_7":{"attribute_name":"著者名よみ","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"43015","nameIdentifierScheme":"WEKO"}],"names":[{"name":"オダ, ユウジ"}]},{"nameIdentifiers":[{"nameIdentifier":"43016","nameIdentifierScheme":"WEKO"}],"names":[{"name":"トノムラ, ケンゾウ"}]}]},"item_1_full_name_8":{"attribute_name":"著者名(英)","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"43017","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ODA, Yuji","nameLang":"en"}]},{"nameIdentifiers":[{"nameIdentifier":"43018","nameIdentifierScheme":"WEKO"}],"names":[{"name":"TONOMURA, Kenzo","nameLang":"en"}]}]},"item_1_source_id_13":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00217655","subitem_source_identifier_type":"NCID"}]},"item_1_text_10":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Food Science and Technology, Fukuyama University"},{"subitem_text_language":"en","subitem_text_value":"Department of Food Science and Technology, Fukuyama University"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"1997-12-01"}],"displaytype":"detail","filename":"KJ00005781390.pdf","filesize":[{"value":"384.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"url":"https://fukuyama-u.repo.nii.ac.jp/record/8020/files/KJ00005781390.pdf"},"version_id":"dd35a83f-78a6-49e3-9fe1-fd06dbcc65cc"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"α-Galactosidase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"enzyme purification","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Trichosporon cutaneum","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Purification and Characterization of α-Galactosidase from the Yeast Trichosporon cutaneum JCM 2947","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Purification and Characterization of α-Galactosidase from the Yeast Trichosporon cutaneum JCM 2947","subitem_title_language":"en"}]},"item_type_id":"1","owner":"3","path":["704"],"pubdate":{"attribute_name":"公開日","attribute_value":"1997-12-01"},"publish_date":"1997-12-01","publish_status":"0","recid":"8020","relation_version_is_last":true,"title":["Purification and Characterization of α-Galactosidase from the Yeast Trichosporon cutaneum JCM 2947"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-19T10:31:53.943286+00:00"}