{"created":"2023-06-19T09:50:35.441675+00:00","id":7498,"links":{},"metadata":{"_buckets":{"deposit":"ab62ea57-43da-474a-bae7-cd03ab016501"},"_deposit":{"created_by":3,"id":"7498","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"7498"},"status":"published"},"_oai":{"id":"oai:fukuyama-u.repo.nii.ac.jp:00007498","sets":["502:506:662:665"]},"author_link":["41136","41148","41137","41145","41138","41144","41147","41140","41143","41146","41149","41139","41141","41135","41142"],"item_1_biblio_info_14":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2004-09","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"47","bibliographicPageStart":"39","bibliographicVolumeNumber":"3","bibliographic_titles":[{"bibliographic_title":"福山大学生命工学部研究年報"},{"bibliographic_title":"Annual report of Faculty of Life Science and Biotechnology, Fukuyama University","bibliographic_titleLang":"en"}]}]},"item_1_creator_6":{"attribute_name":"著者名(日)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"三輪, 泰彦"}],"nameIdentifiers":[{"nameIdentifier":"41135","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"吉水, 正則"}],"nameIdentifiers":[{"nameIdentifier":"41136","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"飯島, 晋次郎"}],"nameIdentifiers":[{"nameIdentifier":"41137","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山岸, 幸正"}],"nameIdentifiers":[{"nameIdentifier":"41138","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"中田, 篤男"}],"nameIdentifiers":[{"nameIdentifier":"41139","nameIdentifierScheme":"WEKO"}]}]},"item_1_description_1":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_1_description_11":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"海産紅藻スサビノリ葉状体から至適pHを7.5～8.0に示すホスファターゼを見いだした。アルカリ性ホスファターゼはグリセロリン酸の欠乏によって誘導合成され、イオン強度に依存する。アルカリ性ホスファターゼをリン酸飢餓条件下で培養した葉状体から均一なタンパク質まで精製した。ホスファターゼは76kDaのサブユニットの2量体からなる。3'-RACE法とオリゴキャップ法で単離したホスファターゼのcDNAクローン(2293 bp)は687アミノ酸残基からなるORFをコードしている。成熟型は658アミノ酸残基(分子量 70,570)の長さである。ノーザン解析からグリセロリン酸飢餓状態で培養した葉状体にアルカリ性ホスファターゼ遺伝子の2,600 baseの転写産物が発現されることが明らかになった。","subitem_description_type":"Other"}]},"item_1_description_12":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"We have found phosphatase giving a pH optimum between 7.5 and 8.0 in the red algae Porphyra yezoensis thalli. The alkaline phosphatase is synthesized in response to glycerophosphate starvation and is ion strength-dependent. The phosphatase has been purified to homogeneity from glycerophosphate-starved thalli. The phosphatase is comprised of two subunits, each having an apparent molecular mass of 76 kDa by SDS-polyacrylamide gel electrophoresis. A cDNA clone of the alkaline phosphatase has been isolated by a combination of 3' rapid amplification of cDNA ends and oligo-capping method from thalli grown under glycerophosphate-deficient conditions. The cDNA contained a 2,293 bp open reading frame encoding 29 residues for signal peptide and 658 residues for the mature form of phosphatase. Northern blot analysis revealed that a single 2,600 base transcript is expressed only in total RNA from glycerophosphate-starved thalli.","subitem_description_type":"Other"}]},"item_1_full_name_7":{"attribute_name":"著者名よみ","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"41140","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ミワ, ヤウスヒコ"}]},{"nameIdentifiers":[{"nameIdentifier":"41141","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ヨシミズ, マサノリ"}]},{"nameIdentifiers":[{"nameIdentifier":"41142","nameIdentifierScheme":"WEKO"}],"names":[{"name":"イイジマ, シンジロウ"}]},{"nameIdentifiers":[{"nameIdentifier":"41143","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ヤマギシ, ユキマサ"}]},{"nameIdentifiers":[{"nameIdentifier":"41144","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ナカタ, アツオ"}]}]},"item_1_full_name_8":{"attribute_name":"著者名(英)","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"41145","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Miwa, Yausuhiko","nameLang":"en"}]},{"nameIdentifiers":[{"nameIdentifier":"41146","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Yoshimizu, Masanori","nameLang":"en"}]},{"nameIdentifiers":[{"nameIdentifier":"41147","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Iijima, Shinjiro","nameLang":"en"}]},{"nameIdentifiers":[{"nameIdentifier":"41148","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Yamagishi, Yukimasa","nameLang":"en"}]},{"nameIdentifiers":[{"nameIdentifier":"41149","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Nakata, Atsuo","nameLang":"en"}]}]},"item_1_source_id_13":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AA11870542","subitem_source_identifier_type":"NCID"}]},"item_1_text_10":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Marine Biotechnology, Faculty of Life Science and Biotechnology Fukuyama University"},{"subitem_text_language":"en","subitem_text_value":"Department of Marine Biotechnology, Faculty of Life Science and Biotechnology Fukuyama University"},{"subitem_text_language":"en","subitem_text_value":"Department of Marine Biotechnology, Faculty of Life Science and Biotechnology Fukuyama University"},{"subitem_text_language":"en","subitem_text_value":"Department of Marine Biotechnology, Faculty of Life Science and Biotechnology Fukuyama University"},{"subitem_text_language":"en","subitem_text_value":"Department of Marine Biotechnology, Faculty of Life Science and Biotechnology Fukuyama University"}]},"item_1_text_9":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_value":"福山大学生命工学部海洋生物工学科"},{"subitem_text_value":"福山大学生命工学部海洋生物工学科"},{"subitem_text_value":"福山大学生命工学部海洋生物工学科"},{"subitem_text_value":"福山大学生命工学部海洋生物工学科"},{"subitem_text_value":"福山大学生命工学部海洋生物工学科"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2004-09-01"}],"displaytype":"detail","filename":"KJ00005782163.pdf","filesize":[{"value":"681.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"url":"https://fukuyama-u.repo.nii.ac.jp/record/7498/files/KJ00005782163.pdf"},"version_id":"81e6434a-cc0c-46b1-a9ba-f73692e1330b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"クローニング","subitem_subject_scheme":"Other"},{"subitem_subject":"ホスファターゼ","subitem_subject_scheme":"Other"},{"subitem_subject":"Porphyra yezoensis","subitem_subject_scheme":"Other"},{"subitem_subject":"cloning","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"phosphatase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Porphyra yezoensis","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"海産紅藻スサビノリのアルカリ性ホスファターゼのcDNAクローニングと発現","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"海産紅藻スサビノリのアルカリ性ホスファターゼのcDNAクローニングと発現"},{"subitem_title":"Cloning and expression of alkaline phosphatase in the red algae Porphyra yezoensis","subitem_title_language":"en"}]},"item_type_id":"1","owner":"3","path":["665"],"pubdate":{"attribute_name":"公開日","attribute_value":"2004-09-01"},"publish_date":"2004-09-01","publish_status":"0","recid":"7498","relation_version_is_last":true,"title":["海産紅藻スサビノリのアルカリ性ホスファターゼのcDNAクローニングと発現"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-19T10:42:02.195963+00:00"}