@article{oai:fukuyama-u.repo.nii.ac.jp:00007487,
 author = {松崎, 浩明 and 丸木, 貴之 and 山根, 祥資 and 福井, 作蔵 and 秦野, 琢之},
 journal = {福山大学生命工学部研究年報, Annual report of Faculty of Life Science and Biotechnology, Fukuyama University},
 month = {Sep},
 note = {P(論文), Saccharomyces cerevisiaeにおいて第III番染色体にPGK-lacZ遺伝子を挿入した場合,その発現量は染色体上の位置で異なり位置効果を示す。発現量はCEN3配列の両側でCEN3配列から数百bpの部位で低かった。それら両部位で,PGK-lacZ遺伝子の転写がCEN3配列からテロメアに向かう方向に挿入した場合の発現量が逆方向の場合よりも低くなり,CEN3配列が発現抑制に働く可能性が示唆された。それら2部位に挟まれた領域で発現抑制に働く領域を限定した結果,CEN3配列が抑制に働いた。さらに,第IV番染色体と第V番染色体のセントロメア近傍でもPGK-lacZ遺伝子の発現量は低下した。これらの結果から,セントロメア配列が遺伝子発現を抑制することが分かった。, There is a possibility of many kinds of chromosomal position effects on gene expression because of chromosomal higher-order structures, nuclear localization of each region of chromosome, and unequal distribution of transcription factors within a nucleus. In our previous study, we have detected chromosomal position effects on the PGK-lacZ gene on chromosome III in Saccharomyces cerevisiae. As one of such position effects, points located near centromere showed significantly lower activity. So, we tried to understand mechanism of position effects near the CEN3 sequence. Points 113 and 114 are located at 0.6 kb from the CEN3 sequence on a left arm and at 0.4 kb on a right arm, respectively. PGK-lacZ genes at those two points showed lower levels of expression. Introduction of the CEN3 sequence (117 bp) near the PGK-lacZ gene on a plasmid conferred lower level of expression on the reporter gene, and introduction of the CEN3-flanking sequence lacking centromere did not. Moreover, expression levels of the PGK-lacZ gene were lower at points located near the centromere of chromosomes V and XV. Therefore, we think that the centromere sequence prevents fully-expression of genes located near the centromere.},
 pages = {1--8},
 title = {Saccharomyces cerevisiaeのセントロメア近傍における遺伝子発現抑制},
 volume = {2},
 year = {2003}
}