{"created":"2023-06-19T09:50:33.614560+00:00","id":7455,"links":{},"metadata":{"_buckets":{"deposit":"bc770738-5867-4386-bb9e-409b5ba6f506"},"_deposit":{"created_by":3,"id":"7455","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"7455"},"status":"published"},"_oai":{"id":"oai:fukuyama-u.repo.nii.ac.jp:00007455","sets":["502:506:653:659"]},"author_link":["41033","41031","41037","41036","41038","41040","41032","41041","41030","41039","41034","41035"],"item_1_biblio_info_14":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1995-12","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"38","bibliographicPageStart":"29","bibliographicVolumeNumber":"6","bibliographic_titles":[{"bibliographic_title":"福山大学附属内海生物資源研究所報告"}]}]},"item_1_creator_6":{"attribute_name":"著者名(日)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"沖増, 英治"}],"nameIdentifiers":[{"nameIdentifier":"41030","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"西尾, 克己"}],"nameIdentifiers":[{"nameIdentifier":"41031","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"芦田, 貴行"}],"nameIdentifiers":[{"nameIdentifier":"41032","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"雨村, 明倫"}],"nameIdentifiers":[{"nameIdentifier":"41033","nameIdentifierScheme":"WEKO"}]}]},"item_1_description_1":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_1_description_11":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"ラット肝ミトコンドリアのエネルギー依存性膜電位変化に応じて,反応液内のイオン濃度が変化する脂溶性イオン(tetraphenyl phosphonium ion, TPP^+)の濃度を選択的に測定できるTPP^+電極を作製し,海洋環境汚染化合物のもたらす影響について検討した。1.作製したTPP^+電極を用いてミトコンドリアのエネルギー状態の変化をその膜電位変化として捉えることができた。2.TBTO添加は,形成される膜電位を消失させる効果として認められた。3.コハク酸基質依存性のADPリン酸化反応で生じる膜電位変化は,α-ケトグルタール酸基質依存性の膜電位変化と比較して,ADP添加後,大きな脱分極に続く過分極方向への膜電位変化が認められたが,TBTOの阻害効果は,後者の場合に強く認められた。4.DNP添加によるプロトン依存性脱共役の膜電位変化には,TBTOは,ほとんど影響を与えなかった。以上の結果より,ミトコンドリア内膜に存在する電子伝達系部位Iへの阻害が推察された。また,TPP^+電極が,海洋汚染化合物の生物毒性モニタリング機器の一つとして有効であることが示唆された。","subitem_description_type":"Other"}]},"item_1_description_12":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"The effect of bis[tris-n-butyltin(IV)]oxide, TBTO, on the energy-linked membrane potential of rat liver mitochondria were studied. The assay of membrane potential was examined by tetraphenyl phosphonium ion (TPP^+)selective electrode. An addition of TBTO induced varing degrees of release of incorporated TPP^+ into the incubation medium (dissipation of membrane potential), and above 0.1 ppm, the release of TPP^+ increased progressively with increasing concentrations. TBTO caused an instantaneous release similar to 2,4-dinitrophenol (DNP). Adenosine diphosphate (ADP) elicited a rapid efflux of accumulated TPP^+ (indicative of depolarization) followed by its reaccumulation (indicative of repolarization). The inhibitory effects of TBTO on this depolarization with a subsequent repolarization in the presence of α-ketoglutarate (NADH-dependent substrate) were more effective than those of the changes of membrane potential in the presence of succinate (FADH_2 dependent substrate). The obtained results show a rotenone-like inhibition of electron transport pathway on NADH-dependent substrate.","subitem_description_type":"Other"}]},"item_1_full_name_7":{"attribute_name":"著者名よみ","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"41034","nameIdentifierScheme":"WEKO"}],"names":[{"name":"オキマス, エイジ"}]},{"nameIdentifiers":[{"nameIdentifier":"41035","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ニシオ, カツミ"}]},{"nameIdentifiers":[{"nameIdentifier":"41036","nameIdentifierScheme":"WEKO"}],"names":[{"name":"アシダ, タカユキ"}]},{"nameIdentifiers":[{"nameIdentifier":"41037","nameIdentifierScheme":"WEKO"}],"names":[{"name":"アメムラ, アキノリ"}]}]},"item_1_full_name_8":{"attribute_name":"著者名(英)","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"41038","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Okimasu, Eiji","nameLang":"en"}]},{"nameIdentifiers":[{"nameIdentifier":"41039","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Nishio, Katsumi","nameLang":"en"}]},{"nameIdentifiers":[{"nameIdentifier":"41040","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Ashida, Takayuki","nameLang":"en"}]},{"nameIdentifiers":[{"nameIdentifier":"41041","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Amemura, Akinori","nameLang":"en"}]}]},"item_1_source_id_13":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN10409073","subitem_source_identifier_type":"NCID"}]},"item_1_text_10":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Research Institute of Marine Bioresources, Fukuyama University"},{"subitem_text_language":"en","subitem_text_value":"Research Institute of Marine Bioresources, Fukuyama University"},{"subitem_text_language":"en","subitem_text_value":"Research Institute of Marine Bioresources, Fukuyama University"},{"subitem_text_language":"en","subitem_text_value":"Research Institute of Marine Bioresources, Fukuyama University"}]},"item_1_text_2":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_text_value":"報文"}]},"item_1_text_3":{"attribute_name":"記事種別(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Original Paper"}]},"item_1_text_9":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_value":"福山大学内海生物資源研究所"},{"subitem_text_value":"福山大学内海生物資源研究所:(現)日本メンテナスエンジニヤリング(株)"},{"subitem_text_value":"福山大学内海生物資源研究所"},{"subitem_text_value":"福山大学内海生物資源研究所"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"1995-12-01"}],"displaytype":"detail","filename":"KJ00005783805.pdf","filesize":[{"value":"378.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"url":"https://fukuyama-u.repo.nii.ac.jp/record/7455/files/KJ00005783805.pdf"},"version_id":"ac686760-b107-44a2-a623-b75edda3c340"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"生体エネルギー産生膜の膜電位形成におよぼす海洋環境汚染化合物(トリアルキルスズ化合物)の影響 : 生体膜電位測定のためのTPP^+電極の応用","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"生体エネルギー産生膜の膜電位形成におよぼす海洋環境汚染化合物(トリアルキルスズ化合物)の影響 : 生体膜電位測定のためのTPP^+電極の応用"},{"subitem_title":"Effect of a Marine Pollutant(TBTO) on Changes in the Membrane Potential of Mitochondria","subitem_title_language":"en"}]},"item_type_id":"1","owner":"3","path":["659"],"pubdate":{"attribute_name":"公開日","attribute_value":"1995-12-01"},"publish_date":"1995-12-01","publish_status":"0","recid":"7455","relation_version_is_last":true,"title":["生体エネルギー産生膜の膜電位形成におよぼす海洋環境汚染化合物(トリアルキルスズ化合物)の影響 : 生体膜電位測定のためのTPP^+電極の応用"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-19T10:42:54.344309+00:00"}