@article{oai:fukuyama-u.repo.nii.ac.jp:00007212,
 author = {植木, 寛},
 journal = {福山大学薬学部研究年報, Annual report of the Faculty of Pharmacy & Pharmaceutical Sciences, Fukuyama University},
 month = {},
 note = {P(論文), Vanadium is an essential trace element for animals owing to its physiological and biochemical activities but it is still unknown whether the element is essential for humans. The effects of vanadate on lipoprotein lipase (LPL), a lipid-metabolizing enzyme, were tested using isolated rat fat pads. Vanadate increased the cellular LPL content through the stimulation of intracellular transport of the enzyme for activation, probably glycosylation. The stimulated release of LPL from the fat pads by vanadate was due to the increase in intracellular Ca^<2+> concentration, leading to the fusion of plasma membrane with vesicle including active LPL. Thus, vanadate appears to differ from both insulin and heparin with regard to the mechanism of action although it shows the insulin and heparin-mimic actions. Leptin is the product of the obese gene and secreted from adipose tissue into the circulation. Vanadate, in contrast to insulin, decreased the cellular leptin content and secretion by the increased degradation via a cAMP / PKA-dependent process involving proteasome activation. In hepatocytes, cAMP phosphodiesterase type 3 was stimulated via the increased mitogen-activated protein kinase activity by vanadate. On the other hand, the stimulation by insulin was dependent on the Akt kinase activation. The effects of vanadate were additive to those of insulin, suggesting that vanadate differes from insulin with regard to a receptor- signaling cascade. Furthermore, vanadate showed the anti-platelet and antithrombin actions, leading to the prolongation of blood clotting time. Thus, vanadate seems to be useful as biological tools and medicines.},
 pages = {1--20},
 title = {バナジウムに魅せられて},
 volume = {20},
 year = {2002}
}