{"created":"2023-06-19T09:50:17.294006+00:00","id":7123,"links":{},"metadata":{"_buckets":{"deposit":"a97bca2c-ec34-4549-b371-fd1ac94a43ce"},"_deposit":{"created_by":3,"id":"7123","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"7123"},"status":"published"},"_oai":{"id":"oai:fukuyama-u.repo.nii.ac.jp:00007123","sets":["502:503:627:644"]},"author_link":["37308","37314","37313","37315","37307","37310","37309","37311","37312"],"item_1_biblio_info_14":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1999","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"75","bibliographicPageStart":"74","bibliographicVolumeNumber":"17","bibliographic_titles":[{"bibliographic_title":"福山大学薬学部研究年報"},{"bibliographic_title":"Annual report of the Faculty of Pharmacy & Pharmaceutical Sciences, Fukuyama University","bibliographic_titleLang":"en"}]}]},"item_1_creator_6":{"attribute_name":"著者名(日)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"井上, 裕文"}],"nameIdentifiers":[{"nameIdentifier":"37307","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小橋, 一彌"}],"nameIdentifiers":[{"nameIdentifier":"37308","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鶴田, 泰人"}],"nameIdentifiers":[{"nameIdentifier":"37309","nameIdentifierScheme":"WEKO"}]}]},"item_1_description_1":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_1_description_11":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"2種の蛍光誘導体化試薬を用いた液体クロマトグラフィー(LC)による血清中Free及び尿中Totalのヒドロキシプロリン(Hyp)及びプロリン(Pro)の同時定量法を開発した。血清中のHyp及びProはo-フタルアルデヒドによる処理後に4-(2-フタルイミジニル)フェニルスルポニルクロライド(Phisyl-Cl)で蛍光誘導体に導いた。加水分解尿中のイミノ酸はo-フタルアルデヒド及びBond Elut C18カラムによる処理後に4-(5,6-ジメトキシ-2-フタルイミジニル)フェニルスルホニルクロライド(DPS-Cl)で蛍光誘導体に導いた。この二つの反応液を混合し、過剰の試薬を除去するためにジクロロメタン抽出を行い、得られる水層(10μl)をLCに注入した。イミノ酸のPhisyl及びDPS誘導体はいずれもEx300nm及びEm400nmにおける蛍光検出でアセトニトリル/リン酸塩緩衝液(5mM,pH2.5)を用いたグラジエント溶出法により逆相系カラムで分離検出された。Hyp及びProの検出限界(S/N=3)は注入量あたりPhisyl誘導体でそれぞれ30及び40fmol、DPS誘導体ではいずれも5fmolであった。日内及び日差の精度(R.S.D.)は血清及び尿中Hyp及びProのいずれにおいても2.77%以下であった。血清及び尿に添加したHyp及びProの回収率は97.6～103.6%であった。本法は高感度で精度が高いことから、血清及び尿中Hyp及びProの同時定量に有用である。","subitem_description_type":"Other"}]},"item_1_description_12":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"A method for the simultaneous determination of free serum and total urinary hydroxyproline (Hyp) and proline (Pro) concentrations by liquid chromatography (LC) using two fluorescent labeling reagents was developed. Serum Hyp and Pro, after treatment with o-phthalaldehyde, were derivatized with 4-(2-phthalimidinyl)phenylsulfonyl chloride (Phisyl-Cl). Imino acids in hydrolysed urine were derivatized with 4-(5,6-dimethoxy-2-phthalimidinyl)phenylsulfonyl chloride (DPS-Cl) after treatment with o-phthalaldehyde and cleanup on a Bond Elut C18 Column. The two reaction mixtures were combined and mixed with dichloromethane to extract the excess of the reagents, and then the aqueous layer (10 μl) was subjected to LC. The Phisyl and DPS derivatives of imino acids were separated on a reversed-phase column by a gradient elution with phosphate buffer (5 mM, pH 2.5) and acetonitrile at 35℃ and detected by fluorescence measurement at 300 nm (excitation) and 400 nm (emission). The detection limits for Hyp and Pro were 30 and 40 fmol/injection, respectively, for Phisyl derivatives and 5 fmol/injection for both DPS derivatives. The within-day and day-to-day relative standard deviations for Hyp and Pro in serum and urine were less than 2.77%. The recoveries of Hyp and Pro added to serum and urine were in the region of 97.6-103.6%. The method is sensitive, gives reproducible results, and is useful for simultaneous determination of serum and urinary Hyp and Pro concentrations.","subitem_description_type":"Other"}]},"item_1_full_name_7":{"attribute_name":"著者名よみ","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"37310","nameIdentifierScheme":"WEKO"}],"names":[{"name":"イノウエ, ヒロフミ"}]},{"nameIdentifiers":[{"nameIdentifier":"37311","nameIdentifierScheme":"WEKO"}],"names":[{"name":"コハシ, カズヤ"}]},{"nameIdentifiers":[{"nameIdentifier":"37312","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ツルタ, ヤスト"}]}]},"item_1_full_name_8":{"attribute_name":"著者名(英)","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"37313","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Inoue, Hirofumi","nameLang":"en"}]},{"nameIdentifiers":[{"nameIdentifier":"37314","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Kohashi, Kazuya","nameLang":"en"}]},{"nameIdentifiers":[{"nameIdentifier":"37315","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Tsuruta, Yasuto","nameLang":"en"}]}]},"item_1_source_id_13":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN10064550","subitem_source_identifier_type":"NCID"}]},"item_1_text_10":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"FACULTY OF PHARMACY & PHARMACEUTICAL SCIENCES FUKUYAMA UNIVERSITY"},{"subitem_text_language":"en","subitem_text_value":"FACULTY OF PHARMACY & PHARMACEUTICAL SCIENCES FUKUYAMA UNIVERSITY"},{"subitem_text_language":"en","subitem_text_value":"FACULTY OF PHARMACY & PHARMACEUTICAL SCIENCES FUKUYAMA UNIVERSITY"}]},"item_1_text_2":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_text_value":"発表論文抄録"}]},"item_1_text_9":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_value":"福山大学薬学部"},{"subitem_text_value":"福山大学薬学部"},{"subitem_text_value":"福山大学薬学部"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"1999-01-01"}],"displaytype":"detail","filename":"KJ00005784722.pdf","filesize":[{"value":"90.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"url":"https://fukuyama-u.repo.nii.ac.jp/record/7123/files/KJ00005784722.pdf"},"version_id":"d49ff2b2-cd23-4765-a511-a7727d7007c1"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"2種類の蛍光誘導体化試薬を用いた血清及び尿中ヒドロキシプロリン及びプロリンの液体クロマトグラフィーによる同時定量","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"2種類の蛍光誘導体化試薬を用いた血清及び尿中ヒドロキシプロリン及びプロリンの液体クロマトグラフィーによる同時定量"},{"subitem_title":"Simultaneous determination of serum and urinary hydroxyproline and proline by liquid chromatography using two fluorescent labeling reagents","subitem_title_language":"en"}]},"item_type_id":"1","owner":"3","path":["644"],"pubdate":{"attribute_name":"公開日","attribute_value":"1999-01-01"},"publish_date":"1999-01-01","publish_status":"0","recid":"7123","relation_version_is_last":true,"title":["2種類の蛍光誘導体化試薬を用いた血清及び尿中ヒドロキシプロリン及びプロリンの液体クロマトグラフィーによる同時定量"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-19T10:49:31.359201+00:00"}