@article{oai:fukuyama-u.repo.nii.ac.jp:00007105, author = {八木, 晟 and 角田, まさ子 and 江草, 太郎 and 赤崎, 健司 and 辻, 宏}, journal = {福山大学薬学部研究年報, Annual report of the Faculty of Pharmacy & Pharmaceutical Sciences, Fukuyama University}, month = {}, note = {P(論文), アロエベラゲルから分画されたヒト正常上皮細胞の増殖活性を示す糖蛋白(ベレクチン)に対する抗体を、兎血清より分画した。この抗体を用いて、アロエベラ、キダチアロエおよびアロエシネンシスゲルの透析内液からえられた高分子画分につき、(1)免疫沈降反応と(2)オクタローニー法とを用いてベレクチン量を定量した。その結果、本抗体にこれらアロエ種の識別に有効であることが証明された。, Verectin antiserum raised in white rabbits was immunoprecipitated with the Aloe vera nondialysable fraction. Analysis of the immunoprecipitation revealed that verectin accounted for about 1.25% of the total proteins in the nondialysable fraction of Aloe vera gel. The verectin antibody showed differential immunoreactivities against nondialysable fractions of A. arborescens, A. chinensis, and A. vera: 1) an immunopreciptin line was formed against the fraction of A. vera, but not against those of A. arborescens and A. chinensis gel in an Ouchterlony double immunodiffusion test and 2) an immunopositive band was detected in the A. vera and A. chinensis nondialysable fractions but not in that of A. arborescens in immunoblotting. These findings indicate that verectin antibody can be used to distinguish Aloe materials.}, title = {アロエベラ、キダチアロエおよびアロエシネンシスの免疫化学的識別法}, volume = {17}, year = {1999} }