@article{oai:fukuyama-u.repo.nii.ac.jp:00007022, author = {高橋, 幸江}, journal = {福山大学薬学部研究年報, Annual report of the Faculty of Pharmacy & Pharmaceutical Sciences, Fukuyama University}, month = {}, note = {P(論文), I report the construction of physical maps of chromosomes for Borrelia miyamotoi HT31 and Borrelia turicatae (relapsing fever agent) by pulsed field gel electrophoresis of DNA fragments generated by digestion of chromosomal DNA with rare-cutting restriction endonucleases and reciprocal hybridization. The size of the B. miyamotoi chromosome was calculated to be approximately 925 kilobase pairs and the chromosome for B. turicatae was estimated to be 951 kilobase pairs. The chromosomes of B. miyamotoi and B. turicatae consisted of single linear molecules. The locations of several genes have been assigned to the chromosome maps by Southern hybridization using specific gene probes. Comparison of the genetic maps of the two species of Borrelia provided evidence that the gene order on the chromosomes is quite similar to that of Borrelia burgdorferi sensu lato strains and is highly conserved in the genus Borrelia.}, pages = {25--42}, title = {新種ボレリアBorrelia miyamotoiの染色体地図}, volume = {15}, year = {1997} }