@article{oai:fukuyama-u.repo.nii.ac.jp:00005316,
 author = {阪本, 憲司 and 河内, 広行 and 沖増, 英治 and 雨村, 明倫},
 journal = {福山大学内海生物資源研究所報告},
 month = {Dec},
 note = {P(論文), 種苗生産の現場では,餌料生物としての微細藻類の大量培養が回分式あるいは半連続式で行われている。回分培養の場合,増殖が対数期中期・後期あるいは静止期に達した時に全培養物を収穫して餌料として供されている。半連続培養は,増殖が対数期中期あるいは後期に達した時に培養物の一部を収穫し,新しい培地を等量補充する培養方式である。この場合,培養は数週間あるいは数カ月間培続けられるが,収穫する培養液の量比,収穫する時間間隔によって増殖相が変化する。回分培養においては対数増殖期と静止期において細胞成分が異なることが種々の餌料微細藻類について報告されており,栄養価の高い細胞を適切な時期に収穫する事の重要性が指摘されている。しかし,半連続培養の場合,植え継ぎ条件(再生率)によって細胞成分がどのように変化するといった研究はほとんど行われていない。本研究では,魚介類の種苗生産の初期餌料に用いられているNannechloropsis oculataをまず回分式で培養し,各増殖相での細胞成分を調べ,ついで半連続培養を行い,再生率を変えることにより細胞成分がどのように変化するかを調べ,どの再生率で得られた細胞が餌料としての栄養価に優れているかについて考察を行った。, The chemical composition (protein, carbohydrate, lipid, and fatty acids) of the marine microalga Nannochloropsis oculata was studied in batch culture at five growth phases (logarithmic, late-logarithmic, transient, early-stationary, and stationary phases) and in semicontinuous culture with four rates of three-day renewal of the culture medium in the range 10-30%. In batch mode, protein and carbohydrate contents of the cells were high from the late-logarithmic to the transient phases and from the transient to the early stationary phases, respectively. On the other hand, content of lipid did not changed significantly with growth phase. The content of fatty acids, especially that of eicosapentaenoic acid (EPA) which is considered to be essential in many marine animal diets for growth and survival rates, increased at the late-logarithmic phase and decreased at the later stages. Thus, the nutritive value of the late-logarithmic-phase culture was evaluated highly because of its high levels of protein, gross fatty acids and EPA. In semicontinuous mode, 30% renewal rate gave a high level of protein content of the cells, the level decreasing with a decease of renewal rate, whereas maximum levels of carbohydrate, lipid, and fatty acids were obtained with 10% reneal rate, although EPA content is the highest with 30% renewal rate. Growth in culture with 30% renewal rate was maintained at the late-logarithmic phase and the maximum production of cells with a high nutritive value was found to be obtained when the cells were cultured and harvested under this condition.},
 pages = {15--23},
 title = {回分培養および異なる再生率での半連続培養で得られたNannochloropsis oculataの栄養価},
 volume = {9},
 year = {1998}
}