WEKO3
インデックスリンク
アイテム
{"_buckets": {"deposit": "5f30bfd6-33d3-4cff-9ddd-547ea9b73e08"}, "_deposit": {"created_by": 18, "id": "8776", "owners": [18], "pid": {"revision_id": 0, "type": "depid", "value": "8776"}, "status": "published"}, "_oai": {"id": "oai:fukuyama-u.repo.nii.ac.jp:00008776", "sets": ["783"]}, "author_link": ["45914", "45910", "45911", "45912", "45915", "45913"], "item_10002_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2013-12-25", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "31", "bibliographicPageEnd": "43", "bibliographicPageStart": "43", "bibliographic_titles": [{"bibliographic_title": "福山大学薬学部研究年報"}, {"bibliographic_title": "Annual report of the Faculty of Pharmacy \u0026 Pharmaceutical Sciences, Fukuyama University", "bibliographic_titleLang": "en"}]}]}, "item_10002_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "\u003csup\u003e13\u003c/sup\u003eC標識テオフィリン(TP)経口投与後のヒト尿中代謝物の\u003csup\u003e13\u003c/sup\u003eC-NMRによる定量を検討した。腎及び肝機能正常な5人の男性被験者に\u003csup\u003e13\u003c/sup\u003eC標識テオフィリン(1,7-ジメチル-\u003csup\u003e13\u003c/sup\u003eC\u003csub\u003e2\u003c/sub\u003e-キサンチン)50mgを投与し,Sep-Pac Vac RCカラムにより固相抽出した。抽出試料より未変化TP及び1,3-ジメチル尿酸(13U),3-メチルキサンチン(3X),1-メチル尿酸(1U)をNMRにより特異的に検出できた。\u003csup\u003e13\u003c/sup\u003eC標識代謝物標品と内部標準として[3-メチル-\u003csup\u003e13\u003c/sup\u003eC]カフェイン(3-\u003csup\u003e13\u003c/sup\u003eC-CF)のシグナル積算値の比はそれらのモル比に比例した。他の代謝物についても同様の関係が成立するものと推定される。\u003csup\u003e13\u003c/sup\u003eC標識TPの尿中代謝物の定量結果より,喫煙者と非喫煙者の間には肝臓のCYP1A2活性を反映した著しい個人差が認められた。薬物代謝研究において,\u003csup\u003e13\u003c/sup\u003eC-NMR法は代謝物の検索及び定量に有用であることが認められた。", "subitem_description_type": "Abstract"}]}, "item_10002_description_6": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "Determination of human urinary metabolites after oral administration of \u003csup\u003e13\u003c/sup\u003eC-labeled theophylline (TP) was examined by \u003csup\u003e13\u003c/sup\u003eC-NMR spectroscopy. \u003csup\u003e13\u003c/sup\u003eC-labeled TP(1,7-dimethyl-\u003csup\u003e13\u003c/sup\u003eC\u003csub\u003e2\u003c/sub\u003e-xanthine)was administered at a dose of 50mg to 5male volunteers who have normal hepatic and renal functions. Urinary samples were prepared by solid phase extraction with Sep-Pac Vac RC columns. NMR spectra of the urinary extracts indicated the specific resonances signals of unchanged TP and its main metabolites, 1, 3-dimethyluric acid(13U), 3-methylxanthine(3X) and 1-methyluric acid(1U). The integral signal intensity ratio of authentic \u003csup\u003e13\u003c/sup\u003eC-labeled metabolites to [3-methyl-\u003csup\u003e13\u003c/sup\u003eC] caffeine(3-\u003csup\u003e13\u003c/sup\u003eC-CF) as an internal standard was directly proportional to the molar ratio of the compounds. Similar relationship was expected to other metabolites. From the results of quantification of urinary metabolites of \u003csup\u003e13\u003c/sup\u003eC-labeled TP, remarkable individual difference was observed in the urinary excretion of metabolites between smoker and nonsmoker due to influence of hepatic CYP1A2 activity. In drug metabolism study, \u003csup\u003e13\u003c/sup\u003eC-NMR spectroscopy with \u003csup\u003e13\u003c/sup\u003eC-tracer was useful technique for detection and quantification of the metabolites.", "subitem_description_type": "Other"}]}, "item_10002_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "45913", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "GOROMARU, Tsuyoshi"}]}, {"nameIdentifiers": [{"nameIdentifier": "45914", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "KAKIHARA, Yoshie"}]}, {"nameIdentifiers": [{"nameIdentifier": "45915", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "MOTOYASHIKI, Toshio"}]}]}, "item_10002_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "福山大学薬学部"}]}, "item_10002_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AN10064550", "subitem_source_identifier_type": "NCID"}]}, "item_10002_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0288-724X", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "五郎丸, 毅"}], "nameIdentifiers": [{"nameIdentifier": "45910", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "柿原, 良枝"}], "nameIdentifiers": [{"nameIdentifier": "45911", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "本屋敷, 敏雄"}], "nameIdentifiers": [{"nameIdentifier": "45912", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-12-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "31-43.pdf", "filesize": [{"value": "135.7 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "Copyright (c) 2013 by Fukuyama University", "licensetype": "license_free", "mimetype": "application/pdf", "size": 135700.0, "url": {"label": "五郎丸毅(発表論文抄録(2012))", "url": "https://fukuyama-u.repo.nii.ac.jp/record/8776/files/31-43.pdf"}, "version_id": "87570c88-90f4-4afc-a6ef-712eec6bcb7f"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "determination", "subitem_subject_scheme": "Other"}, {"subitem_subject": "urinary metabolites", "subitem_subject_scheme": "Other"}, {"subitem_subject": "¹³C-labeledtheophylline", "subitem_subject_scheme": "Other"}, {"subitem_subject": "NMR", "subitem_subject_scheme": "Other"}, {"subitem_subject": "determination", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "urinary metabolites", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "¹³C-labeledtheophylline", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "NMR", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "^\u003c13\u003eC標識テオフィリン服用後のヒト尿中代謝物のNMRによる定量(発表論文抄録(2012))", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "^\u003c13\u003eC標識テオフィリン服用後のヒト尿中代謝物のNMRによる定量(発表論文抄録(2012))"}, {"subitem_title": "Determination of Human Urinary Metabolites by NMR after Administration of ^\u003c13\u003eC-labeled Theophylline", "subitem_title_language": "en"}]}, "item_type_id": "10002", "owner": "18", "path": ["783"], "permalink_uri": "https://fukuyama-u.repo.nii.ac.jp/records/8776", "pubdate": {"attribute_name": "公開日", "attribute_value": "2017-12-20"}, "publish_date": "2017-12-20", "publish_status": "0", "recid": "8776", "relation": {}, "relation_version_is_last": true, "title": ["^\u003c13\u003eC標識テオフィリン服用後のヒト尿中代謝物のNMRによる定量(発表論文抄録(2012))"], "weko_shared_id": -1}
^<13>C標識テオフィリン服用後のヒト尿中代謝物のNMRによる定量(発表論文抄録(2012))
https://fukuyama-u.repo.nii.ac.jp/records/8776
https://fukuyama-u.repo.nii.ac.jp/records/87760ecdcdb7-3c39-4fd7-8feb-829b82c2bdd4
名前 / ファイル | ライセンス | アクション |
---|---|---|
五郎丸毅(発表論文抄録(2012)) (135.7 kB)
|
Copyright (c) 2013 by Fukuyama University
|
Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2017-12-20 | |||||
タイトル | ||||||
タイトル | ^<13>C標識テオフィリン服用後のヒト尿中代謝物のNMRによる定量(発表論文抄録(2012)) | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Determination of Human Urinary Metabolites by NMR after Administration of ^<13>C-labeled Theophylline | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | determination | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | urinary metabolites | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ¹³C-labeledtheophylline | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | NMR | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | determination | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | urinary metabolites | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | ¹³C-labeledtheophylline | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | NMR | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
五郎丸, 毅
× 五郎丸, 毅× 柿原, 良枝× 本屋敷, 敏雄 |
|||||
著者別名 | ||||||
識別子Scheme | WEKO | |||||
識別子 | 45913 | |||||
姓名 | GOROMARU, Tsuyoshi | |||||
著者別名 | ||||||
識別子Scheme | WEKO | |||||
識別子 | 45914 | |||||
姓名 | KAKIHARA, Yoshie | |||||
著者別名 | ||||||
識別子Scheme | WEKO | |||||
識別子 | 45915 | |||||
姓名 | MOTOYASHIKI, Toshio | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | <sup>13</sup>C標識テオフィリン(TP)経口投与後のヒト尿中代謝物の<sup>13</sup>C-NMRによる定量を検討した。腎及び肝機能正常な5人の男性被験者に<sup>13</sup>C標識テオフィリン(1,7-ジメチル-<sup>13</sup>C<sub>2</sub>-キサンチン)50mgを投与し,Sep-Pac Vac RCカラムにより固相抽出した。抽出試料より未変化TP及び1,3-ジメチル尿酸(13U),3-メチルキサンチン(3X),1-メチル尿酸(1U)をNMRにより特異的に検出できた。<sup>13</sup>C標識代謝物標品と内部標準として[3-メチル-<sup>13</sup>C]カフェイン(3-<sup>13</sup>C-CF)のシグナル積算値の比はそれらのモル比に比例した。他の代謝物についても同様の関係が成立するものと推定される。<sup>13</sup>C標識TPの尿中代謝物の定量結果より,喫煙者と非喫煙者の間には肝臓のCYP1A2活性を反映した著しい個人差が認められた。薬物代謝研究において,<sup>13</sup>C-NMR法は代謝物の検索及び定量に有用であることが認められた。 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Determination of human urinary metabolites after oral administration of <sup>13</sup>C-labeled theophylline (TP) was examined by <sup>13</sup>C-NMR spectroscopy. <sup>13</sup>C-labeled TP(1,7-dimethyl-<sup>13</sup>C<sub>2</sub>-xanthine)was administered at a dose of 50mg to 5male volunteers who have normal hepatic and renal functions. Urinary samples were prepared by solid phase extraction with Sep-Pac Vac RC columns. NMR spectra of the urinary extracts indicated the specific resonances signals of unchanged TP and its main metabolites, 1, 3-dimethyluric acid(13U), 3-methylxanthine(3X) and 1-methyluric acid(1U). The integral signal intensity ratio of authentic <sup>13</sup>C-labeled metabolites to [3-methyl-<sup>13</sup>C] caffeine(3-<sup>13</sup>C-CF) as an internal standard was directly proportional to the molar ratio of the compounds. Similar relationship was expected to other metabolites. From the results of quantification of urinary metabolites of <sup>13</sup>C-labeled TP, remarkable individual difference was observed in the urinary excretion of metabolites between smoker and nonsmoker due to influence of hepatic CYP1A2 activity. In drug metabolism study, <sup>13</sup>C-NMR spectroscopy with <sup>13</sup>C-tracer was useful technique for detection and quantification of the metabolites. | |||||
書誌情報 |
福山大学薬学部研究年報 en : Annual report of the Faculty of Pharmacy & Pharmaceutical Sciences, Fukuyama University 号 31, p. 43-43, 発行日 2013-12-25 |
|||||
出版者 | ||||||
出版者 | 福山大学薬学部 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0288-724X | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN10064550 |