WEKO3
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血清中パロキセチンの簡便な前処理による蛍光プレラベルHPLC分析法(発表論文抄録(2013))
https://fukuyama-u.repo.nii.ac.jp/records/8740
https://fukuyama-u.repo.nii.ac.jp/records/874082a59914-96b1-4160-a936-363b8e260f39
名前 / ファイル | ライセンス | アクション |
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井上裕文(発表論文抄録(2013)) (194.2 kB)
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Copyright (c) 2014 by Fukuyama University
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2017-12-19 | |||||
タイトル | ||||||
タイトル | 血清中パロキセチンの簡便な前処理による蛍光プレラベルHPLC分析法(発表論文抄録(2013)) | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Determination of paroxetine in serum treated with simple pretreatment by pre-column high-performance liquid chromatography using 4-(5,6-dimethoxy-2-phthalimidinyl)-2-methoxyphenylsulfonyl chloride as a fluorescent labeling reagent. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Adult | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Chromatography, High Pressure Liquid | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Female | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Fluorescent Dyes | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Humans | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Limit of Detection | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Male | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Middle Aged | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Paroxetine | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Phthalimides | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Reproducibility of Results | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
Inoue, Hirofumi
× Inoue, Hirofumi× Harada, Takuya× Eto, Seiji× Nakashima, Ken× Ibusuki, Takuya× Kosha, Keiko× Date, Yuuko× Sanematsu, Emiko× Shinohara, Yoshitake× Takahashi, Kojiro× Yoshimura, Reiji× Nakamura, Jun× Kojima, Eijiro× Tsuruta, Yasuto |
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著者別名 | ||||||
識別子Scheme | WEKO | |||||
識別子 | 45108 | |||||
識別子Scheme | CiNii ID | |||||
識別子URI | http://ci.nii.ac.jp/nrid/9000006296797 | |||||
識別子 | 9000006296797 | |||||
姓名 | 井上, 裕文 | |||||
著者別名 | ||||||
識別子Scheme | WEKO | |||||
識別子 | 45665 | |||||
姓名 | 原田, 卓也 | |||||
著者別名 | ||||||
識別子Scheme | WEKO | |||||
識別子 | 45338 | |||||
識別子Scheme | CiNii ID | |||||
識別子URI | http://ci.nii.ac.jp/nrid/9000002858230 | |||||
識別子 | 9000002858230 | |||||
姓名 | 江藤, 精二 | |||||
著者別名 | ||||||
識別子Scheme | WEKO | |||||
識別子 | 45666 | |||||
姓名 | 中嶋, 研 | |||||
著者別名 | ||||||
識別子Scheme | WEKO | |||||
識別子 | 45667 | |||||
姓名 | 指宿, 卓也 | |||||
著者別名 | ||||||
識別子Scheme | WEKO | |||||
識別子 | 45668 | |||||
識別子Scheme | CiNii ID | |||||
識別子URI | http://ci.nii.ac.jp/nrid/9000342904965 | |||||
識別子 | 9000342904965 | |||||
姓名 | 古謝, 景子 | |||||
著者別名 | ||||||
識別子Scheme | WEKO | |||||
識別子 | 45337 | |||||
識別子Scheme | CiNii ID | |||||
識別子URI | http://ci.nii.ac.jp/nrid/9000005189599 | |||||
識別子 | 9000005189599 | |||||
姓名 | 伊達, 有子 | |||||
著者別名 | ||||||
識別子Scheme | WEKO | |||||
識別子 | 45669 | |||||
識別子Scheme | CiNii ID | |||||
識別子URI | http://ci.nii.ac.jp/nrid/9000279907278 | |||||
識別子 | 9000279907278 | |||||
姓名 | 實松, 絵美子 | |||||
著者別名 | ||||||
識別子Scheme | WEKO | |||||
識別子 | 45670 | |||||
識別子Scheme | CiNii ID | |||||
識別子URI | http://ci.nii.ac.jp/nrid/9000002858586 | |||||
識別子 | 9000002858586 | |||||
姓名 | 篠原, 義剛 | |||||
著者別名 | ||||||
識別子Scheme | WEKO | |||||
識別子 | 45671 | |||||
識別子Scheme | CiNii ID | |||||
識別子URI | http://ci.nii.ac.jp/nrid/9000002858591 | |||||
識別子 | 9000002858591 | |||||
姓名 | 高橋, 浩二郎 | |||||
著者別名 | ||||||
識別子Scheme | WEKO | |||||
識別子 | 45672 | |||||
識別子Scheme | CiNii ID | |||||
識別子URI | http://ci.nii.ac.jp/nrid/1000090248568 | |||||
識別子 | 1000090248568 | |||||
姓名 | 吉村, 玲児 | |||||
著者別名 | ||||||
識別子Scheme | WEKO | |||||
識別子 | 45673 | |||||
識別子Scheme | CiNii ID | |||||
識別子URI | http://ci.nii.ac.jp/nrid/1000030130876 | |||||
識別子 | 1000030130876 | |||||
姓名 | 中村, 純 | |||||
著者別名 | ||||||
識別子Scheme | WEKO | |||||
識別子 | 45674 | |||||
識別子Scheme | CiNii ID | |||||
識別子URI | http://ci.nii.ac.jp/nrid/9000345371922 | |||||
識別子 | 9000345371922 | |||||
姓名 | 小嶋, 英二朗 | |||||
著者別名 | ||||||
識別子Scheme | WEKO | |||||
識別子 | 45106 | |||||
識別子Scheme | CiNii ID | |||||
識別子URI | http://ci.nii.ac.jp/nrid/9000005174471 | |||||
識別子 | 9000005174471 | |||||
姓名 | 鶴田, 泰人 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The therapeutic drug monitoring of paroxetine could be used to optimize the pharmacological treatment of depressed patients. A simple and sensitive high-performance liquid chromatography procedure was developed for the determination of paroxetine in serum. After simple pretreatment of serum (50 μL) with acetonitrile and o-phthalaldehyde, paroxetine was derivatized with 4-(5,6-dimethoxy-2-phthalimidinyl)-2-methoxyphenylsulfonyl chloride at 70°C for 20 min in borate buffer (0.1 mol/L, pH 8.0) to produce a fluorescent product. The derivative was separated on a reversed-phase column at 40°C for stepwise elution with (A) acetic acid (10 mmol/L) and (B) acetonitrile. The flow rate was 1.0 mL/min. The fluorescence intensity was monitored at excitation and emission wavelengths of 320 and 400 nm, respectively. The within-day and day-to-day relative standard deviations were 3.0-3.4 and 2.7-8.3%, respectively. The detection limit of paroxetine was 8.3 fmol at a signal-to-noise ratio of 3. As the proposed method that only requires a small quantity of serum (50 μL) is simple, sensitive and reproducible, it would be useful for clinical and biochemical research as well as drug monitoring. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | The therapeutic drug monitoring of paroxetine could be used to optimize the pharmacological treatment of depressed patients. A simple and sensitive high-performance liquid chromatography procedure was developed for the determination of paroxetine in serum. After simple pretreatment of serum (50 μL) with acetonitrile and o-phthalaldehyde, paroxetine was derivatized with 4-(5,6-dimethoxy-2-phthalimidinyl)-2-methoxyphenylsulfonyl chloride at 70°C for 20 min in borate buffer (0.1 mol/L, pH 8.0) to produce a fluorescent product. The derivative was separated on a reversed-phase column at 40°C for stepwise elution with (A) acetic acid (10 mmol/L) and (B) acetonitrile. The flow rate was 1.0 mL/min. The fluorescence intensity was monitored at excitation and emission wavelengths of 320 and 400 nm, respectively. The within-day and day-to-day relative standard deviations were 3.0-3.4 and 2.7-8.3%, respectively. The detection limit of paroxetine was 8.3 fmol at a signal-to-noise ratio of 3. As the proposed method that only requires a small quantity of serum (50 μL) is simple, sensitive and reproducible, it would be useful for clinical and biochemical research as well as drug monitoring. | |||||
書誌情報 |
福山大学薬学部研究年報 en : Annual report of the Faculty of Pharmacy & Pharmaceutical Sciences, Fukuyama University 号 32, p. 29-30, 発行日 2014-12-25 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0288-724X | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN10064550 |